SPECIFIC INTERACTION OF THE NEW FLUORESCENT DYE 10-N-NONYL ACRIDINE-ORANGE WITH INNER MITOCHONDRIAL-MEMBRANE - A LIPID-MEDIATED INHIBITION OF OXIDATIVE-PHOSPHORYLATION

被引：56

作者：

MAFTAH, A ^{[1
]}

PETIT, JM ^{[1
]}

JULIEN, R ^{[1
]}

机构：

[1] FAC SCI LIMOGES, GENIUS UNITE BIOTECHNOL, 123 AVE ALBERT THOMAS, F-87060 LIMOGES, FRANCE

来源：

FEBS LETTERS | 1990年 / 260卷 / 02期

关键词：

(Rat liver mitochondria); ATP synthesis; Nonyl acridine orange; Respiratory chain; mitochondria;

D O I：

10.1016/0014-5793(90)80112-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fluorescent dye 10-N-nonyl acridine orange (NAO), known as specifically associated with mitochondria, has been reported to have a cytotoxic effect when high doses were applied to cells. Presently, the biochemical basis of its toxicity was investigated on isolated rat liver mitochondria. At low concentrations, NAO strongly inhibited state 3 respiration and ATP synthesis. At high concentrations, electron transport, ATP hydrolysis, Pi-transport and adenine nucleotide activities were also decreased. All these inhibitions can be explained by probe-cardiolipin interactions which could induce the collapse of energy conversion and/or the modification of membrane fluidity. © 1990.

引用

页码：236 / 240

页数：5

共 29 条

[21] ROGERS KS, 1973, J BIOL CHEM, V248, P7142
[22] CORRELATION BETWEEN LOSSES OF MITOCHONDRIAL ATPASE ACTIVITY AND CARDIOLIPIN DEGRADATION
SANTIAGO, E
LOPEZMOR.N
SEGOVIA, JL
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 53 (02) : 439 - 445
[23] HYDROPHOBIC ACRIDINE-DYES FOR FLUORESCENCE STAINING OF MITOCHONDRIA IN LIVING CELLS .3. SPECIFIC ACCUMULATION OF THE FLUORESCENCE DYE NAO AT THE MITOCHONDRIAL-MEMBRANES IN HELA-CELLS BY HYDROPHOBIC INTERACTION - DECREASE OF RESPIRATORY ACTIVITY AND CHANGES IN THE ULTRASTRUCTURE OF MITOCHONDRIA BY NAO - INCREASE OF FLUORESCENCE INTENSITY OF NAO STAINED MITOCHONDRIA INSITU BY IRRADIATIONA
SEPTINUS, M
BERTHOLD, T
NAUJOK, A
ZIMMERMANN, HW
[J]. HISTOCHEMISTRY, 1985, 82 (01) : 51 - 66
[24] SKULACHEV VP, 1969, FEBS S, V17, P275
[25] SUMMER JB, 1944, SCIENCE, V100, P155
[26] UNCOUPLING OF OXIDATIVE-PHOSPHORYLATION BY DIVALENT CATIONIC CYANINE DYE - PARTICIPATION OF PHOSPHATE TRANSPORTER
TERADA, H
NAGAMUNE, H
MORIKAWA, N
IKUNO, M
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 807 (02) : 168 - 176
[27] Vignais P. V., 1985, STRUCTURE PROPERTIES, V2, P139
[28] ATPASE OF ESCHERICHIA-COLI - PURIFICATION, DISSOCIATION, AND RECONSTITUTION OF ACTIVE COMPLEX FROM ISOLATED SUBUNITS
VOGEL, G
STEINHART, R
[J]. BIOCHEMISTRY, 1976, 15 (01) : 208 - 216
[29] Wharton DC, 1967, METHOD ENZYMOL, V10, P245

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