肝癌患者血清中miR－221、miR－222表达分析

被引：1
|
作者：
张洪亮 [1 ]
杨海霞 [2 ]
赵国强 [3 ]
机构：
[1] 河南省省直第二医院
[2] 新密市郑州新华医院
[3] 郑州大学基础医学院
来源：
中国实用医刊 | 2015年 / 42卷 / 20期
关键词：
肝癌; 血清; miR－221; miR－222; 表达;
D O I：
暂无
中图分类号：
R735.7 [肝肿瘤];
学科分类号：
摘要：
目的探索肝癌患者血清中miR-221 、miR-222表达情况。方法选取2013年至2014年于河南省省直第二医院、郑州大学第二附属医院、河南省人民医院行原发肝癌手术切除患者31例, 同时选取健康体检者68例作为对照;采外周血2 ml,提取RNA, 定量逆转录PCR方法检测miR-221和miR-222相对表达水平。结果健康对照组和肝癌组血清中miR-221和miR-222相对表达量分别为1.92±0.67、1.24±0.36和10.18±2.27、7.57±1.26;肝癌患者血清中miR-221和miR-222相对表达量明显高于正常对照, 差异有统计学意义(P<0.01)。结论肝癌患者血清中miR-221和miR-222相对表达量显著升高, 于肝癌临床上有早期诊疗意义。
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