LncRNA IDH1-AS1 sponges miR-518c-5p to suppress proliferation of epithelial ovarian cancer cell by targeting RMB47

被引：0

作者：

Juan Zhou ^{[1
]}

Yiran Xu ^{[1
]}

Luyao Wang ^{[1
]}

Yu Cong ^{[1
]}

Ke Huang ^{[1
]}

Xinxing Pan ^{[1
]}

Guangquan Liu ^{[1
]}

Wenqu Li ^{[1
]}

Chenchen Dai ^{[1
]}

Pengfei Xu ^{[2
]}

Xuemei Jia ^{[1
]}

机构：

[1] Department of Gynecology, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital

[2] Nanjing Maternity and Child Health Medical Institute, Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital

来源：

The Journal of Biomedical Research | 2024年 / 38卷 / 01期

关键词：

D O I：

暂无

中图分类号：

R737.31 [卵巢肿瘤];

学科分类号：

100214 ;

摘要：

Long noncoding RNA(lnc RNA) IDH1 antisense RNA 1(IDH1-AS1) is involved in the progression of multiple cancers, but its role in epithelial ovarian cancer(EOC) is unknown. Therefore, we investigated the expression levels of IDH1-AS1 in EOC cells and normal ovarian epithelial cells by quantitative real-time PCR(qPCR). We first evaluated the effects of IDH1-AS1 on the proliferation, migration, and invasion of EOC cells through cell counting kit-8, colony formation, Ed U, transwell, wound-healing, and xenograft assays. We then explored the downstream targets of IDH1-AS1 and verified the results by a dual-luciferase reporter, qPCR, rescue experiments,and Western blotting. We found that the expression levels of IDH1-AS1 were lower in EOC cells than in normal ovarian epithelial cells. High IDH1-AS1 expression of EOC patients from the Gene Expression Profiling Interactive Analysis database indicated a favorable prognosis, because IDH1-AS1 inhibited cell proliferation and xenograft tumor growth of EOC. IDH1-AS1 sponged miR-518c-5p whose overexpression promoted EOC cell proliferation. The miR-518c-5p mimic also reversed the proliferation-inhibiting effect induced by IDH1-AS1overexpression. Furthermore, we found that RNA binding motif protein 47(RBM47) was the downstream target of miR-518c-5p, that upregulation of RBM47 inhibited EOC cell proliferation, and that RBM47 overexpressing plasmid counteracted the proliferation-promoting effect caused by the IDH1-AS1 knockdown. Taken together,IDH1-AS1 may suppress EOC cell proliferation and tumor growth via the miR-518c-5p/RBM47 axis.

引用

页码：51 / 65

页数：15