Evaluation of the bactericidal potential of 2780-nm ER,CR:YSGG and 940-nm diode lasers in the root canal system

被引：0

作者：

Erben P. ^{[1
]}

Chang A.M. ^{[2
]}

Darveau R.P. ^{[3
]}

Fong H. ^{[4
]}

Johnson J.D. ^{[1
]}

Paranjpe A. ^{[1
]}

机构：

[1] School of Dentistry, Department of Endodontics, University of Washington, 1959 NE Pacific Street, D-669 Health Science Center, Box 357448, Seattle, 98195, WA

[2] Department of Oral Health Sciences, University of Washington, Seattle, WA

[3] Department of Periodontics, University of Washington, Seattle, WA

[4] Department of Material Science and Engineering, University of Washington, Seattle, WA

来源：

Lasers in Dental Science | 2019年 / 3卷 / 2期

基金：

美国国家卫生研究院;

关键词：

Bacteria; Disinfection; Dual laser; Er; Cr:YSGG; Irrigation;

D O I：

10.1007/s41547-019-00060-z

中图分类号：

学科分类号：

摘要：

Purpose: Successful endodontic therapy relies on chemomechanical debridement. Laser-assisted irrigation techniques are available for debridement. However, there are limited studies demonstrating the effectiveness of dual lasers for root canal disinfection. Hence, the aim of the present study is to determine if laser activation irrigation using the Er,Cr:YSGG and 940-nm diode laser is as effective as positive pressure needle irrigation using sodium hypochlorite (NaOCl) and ethylenediamine tetraacetic acid (EDTA). Methods: Fifty-two caries-free premolars were divided into six groups: G1, sterile water via positive pressure irrigation; G2, EDTA and NaOCl via positive pressure irrigation; G3, sterile water and the Er,Cr:YSGG cleaning and disinfection step; G4, sterile water and the Er,Cr:YSGG cleaning and disinfection step followed by disinfection with the 940-nm diode; G5, sterile water and the Er,Cr:YSGG cleaning step followed by a disinfection step with the Er,Cr:YSGG and diode laser together; and G6, negative controls. S1 samples were validated in both negative and positive control groups via culturing. All teeth, except the negative controls, were inoculated with Enterococcus faecalis. S2 sampling after treatments was completed and tested for bacterial presence via culturing and SEM and CLSM analysis. Results: The data demonstrated that the traditional irrigation group (G2) and the dual laser group (G5) showed the greatest percentage decrease in bacterial counts from the S1 to the S2 group. Conclusions: The data demonstrates that the combination of the Er,Cr:YSGG and 940-nm diode laser wavelengths is safe and more effective than either laser alone and is comparable to needle irrigation with sodium hypochlorite and EDTA. © 2019, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

引用

页码：137 / 146

页数：9

共 46 条

[1]

Peters O.A., Peters C.I., Schonenberger K., Barbakow F., ProTaper rotary root canal preparation: effects of canal anatomy on final shape analysed by micro CT, Int Endod J, 36, 2, pp. 86-92, (2003)

[2]

Torabinejad M., Handysides R., Khademi A.A., Bakland L.K., Clinical implications of the smear layer in endodontics: a review, Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 94, 6, pp. 658-666, (2002)

[3]

Mohammadi Z., Sodium hypochlorite in endodontics: an update review, Int Dent J, 58, 6, pp. 329-341, (2008)

[4]

Gutarts R., Nusstein J., Reader A., Beck M., In vivo debridement efficacy of ultrasonic irrigation following hand-rotary instrumentation in human mandibular molars, J Endod, 31, 3, pp. 166-170, (2005)

[5]

Sabins R.A., Johnson J.D., Hellstein J.W., A comparison of the cleaning efficacy of short-term sonic and ultrasonic passive irrigation after hand instrumentation in molar root canals, J Endod, 29, 10, pp. 674-678, (2003)

[6]

Akcay M., Arslan H., Mese M., Durmus N., Capar I.D., Effect of photon-initiated photoacoustic streaming, passive ultrasonic, and sonic irrigation techniques on dentinal tubule penetration of irrigation solution: a confocal microscopic study, Clin Oral Investig, 21, pp. 2205-2212, (2016)

[7]

Ayranci L.B., Arslan H., Akcay M., Capar I.D., Gok T., Saygili G., Effectiveness of laser-assisted irrigation and passive ultrasonic irrigation techniques on smear layer removal in middle and apical thirds, Scanning, 38, 2, pp. 121-127, (2016)

[8]

Aminoshariae A., Kulild J., Master apical file size—smaller or larger: a systematic review of microbial reduction, Int Endod J, 48, 11, pp. 1007-1022, (2015)

[9]

Lan W.H., Liu H.C., Treatment of dentin hypersensitivity by Nd:YAG laser, J Clin Laser Med Surg, 14, 2, pp. 89-92, (1996)

[10]

Moritz A., Schoop U., Goharkhay K., Sperr W., The CO2 laser as an aid in direct pulp capping, J Endod, 24, 4, pp. 248-251, (1998)

← 1 2 3 4 5 →