Comparative study on the petroleum crude oil degradation potential of microbes from petroleum-contaminated soil and non-contaminated soil

被引:0
作者
T. Ra
Y. Zhao
M. Zheng
机构
[1] Northwest University,
来源
International Journal of Environmental Science and Technology | 2019年 / 16卷
关键词
Bioremediation; 2, 6-Dichlorophenol indophenol; Gravimetric analysis; Hydrocarbon; Microbial metabolism; Oil pollution;
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中图分类号
学科分类号
摘要
Comparative study on the petroleum crude oil degradation potential of microbes from petroleum-contaminated soil and non-contaminated soil was performed in the present paper. Three samples were employed to conduct the evaluation, of which two are from the petroleum-contaminated soil of Yanchang oil field, and one is from non-contaminated soil of Northwest University Campus, Xi’an. The bacterial plate counts in sample No. 1 of the petroleum-contaminated soil were 6.0 × 102 and the bacterial plate counts in sample No. 2 of the petroleum-contaminated soil were 4.0 × 103, while the bacterial plate counts in sample No. 3 of non-petroleum-contaminated were 6.0 × 104. Petroleum-degrading microbes were isolated from Bushnell Haas mineral salt medium and 16 bacterial strains and two fungal strains from PCS sample No. 1, five bacterial strains and one fungal strain from PCS sample No. 2, and 17 bacterial strains and one fungal strain from garden soil, respectively. For primary screening of potential petroleum-degrading microbes, 2, 6-dichlorophenol indophenol test was carried out and nine bacterial strains from PCS No. 1 and four bacterial strains from garden soil showed the potential petroleum-degrading activity. The petroleum degradation percentage of mixture of 13 bacteria was 47.57%, and it was higher than that of four fungi and the combined mixture (13 bacteria and four fungi). Study showed that the microbes isolated from non-contaminated soil had the good petroleum degradation potential.
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页码:7127 / 7136
页数:9
相关论文
共 52 条
[11]  
Arpita G(2006)Marine microorganisms make a meal of oil Nat Rev Microbiol 4 173-182
[12]  
Sheetal S(2011)Screening of petroleum degrading bacteria from cow dung Res J Agri Sci 2 69-71
[13]  
Bharti P(2008)Phylogenetic analysis of long-chain hydrocarbondegradation bacteria and evaluation of their hydrocarbon–degradation by the 2,6-DCPIP assay Biodegradation 19 749-757
[14]  
Irafan M(2013)Isolation, identification and characterization of oil degrading bacteria isolated from contaminated sites of Barmer, Rajasthan Int J Biotechnol Bioeng Res 4 429-436
[15]  
Chukwura EI(2013)Hydrocarbonoclastic bacteria isolated from petroleum contaminated sites in Tunisia; isolation, identification and characterization of the biotechnological potential New Biotechnol 30 723-733
[16]  
Das N(2013)Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek Bioinformation 9 150-157
[17]  
Chandran P(2012)Evolution of crude oil degradation by Chem Eng Trans 27 223-228
[18]  
Ganesh A(2012)Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils Iran J Environ Health Sci Eng 9 1-8
[19]  
Lin J(2006)Remediation of petroleum hydrocarbon polluted systems: exploiting the bioremediation strategies Afr J Biotechnol 5 2520-2525
[20]  
Head IM(2014)Aerobic degradation of petroleum components by microbial consortia J Pet Environ Biotechnol 5 1000195-8