White spot syndrome virus Manipulates Ubiquitin Gene Expression in Penaeus monodon

被引：0
作者：
R. Vidya
P. Gireesh-Babu
K. Pani Prasad
机构：
[1] ICAR,Division of Aquatic Environment and Health Management, Central Institute of Fisheries Education
[2] Indian Council of Agricultural Research,Division of Fish Genetics and Biotechnology, Central Institute of Fisheries Education
来源：
Indian Journal of Virology | 2013年 / 24卷
关键词：
Ubiquitin; Expression profiling; WSSV; Semi-quantitative RT-PCR; ELISA;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The present study reports the effect of WSSV on the expression profiles of shrimp ubiquitin gene both at protein and transcript levels at different time points after infection. A gradual decrease in ubiquitin protein levels was observed till 12 hpi (0.25 and 0.23 μg/mg of the total protein in gills and muscle, respectively) with a sudden increase at 18 hpi (1.86 and 1.8 μg/mg of total protein in gills and muscle, respectively). The RT-PCR results showed a gradual decrease in ubiquitin mRNA levels in gill tissue till 12 hpi while a more or less constant mRNA levels were observed in muscle tissue till 12 hpi. However, a fourfold and 16 fold induction in ubiquitin mRNA levels at 18 hpi in gill and muscle, respectively suggests its possible regulation by WSSV in the manifestation of the disease.
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页码：82 / 84
页数：2
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