Interaction of DNA with sperm-specific histones of the H1 family

被引：0

作者：

Chikhirzhina E.V. ^{[1
]}

Starkova T.Y. ^{[2
]}

Kostyleva E.I. ^{[1
]}

Chikhirzhina G.I. ^{[3
]}

Vorobiev V.I. ^{[1
]}

Polyanichko A.M. ^{[1
,2
]}

机构：

[1] Institute of Cytology, Russian Academy of Sciences, St. Petersburg

[2] Department of Molecular Biophysics, Faculty of Physics, St. Petersburg State University, St. Petersburg

[3] Department of Biochemistry, Faculty of Biology, St. Petersburg State University, St. Petersburg

来源：

Cell and Tissue Biology | 2011年 / 5卷 / 6期

基金：

俄罗斯基础研究基金会;

关键词：

circular dichroism; DNA; DNA melting; DNA-protein interactions; sperm-specific histone H1;

D O I：

10.1134/S1990519X11060058

中图分类号：

学科分类号：

摘要：

Interactions of DNA with sperm-specific histones of the H1 family of sea urchin Strongylocentrotus intermedius, sea star Aphelasterias japonica, and bivalve mollusc Chlamis islandicus were studied using circular dichroism and the DNA melting analysis. Under physiological conditions, the highest DNA compacting ability was found in the echinoderm sperm H1 protein, in which additional α-helical domains are present in their C-terminal sequence. The derivative melting curves have two peaks: the low-temperature peak corresponds to the melting of free DNA, whereas the DNA regions bound to the protein melt at higher temperature. The highest stabilizing ability is characteristic of complexes with the mollusc sperm H1 protein. © 2011 Pleiades Publishing, Ltd.

引用

页码：536 / 542

页数：6

共 31 条

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