PAH utilization by Pseudomonas rhodesiae KK1 isolated from a former manufactured-gas plant site

被引：0

作者：

H.-Y. Kahng

K. Nam

J. Kukor

B.-J. Yoon

D.-H. Lee

D.-C. Oh

S.-K. Kam

K.-H. Oh

机构：

[1] Department of Environmental Education,

[2] College of Education,undefined

[3] Sunchon National University,undefined

[4] Sunchon 540-742,undefined

[5] Korea,undefined

[6] Department of Environmental Education,undefined

[7] Research Institute for Basic Sciences,undefined

[8] Cheju National University,undefined

[9] Jeju 690-756,undefined

[10] Korea,undefined

[11] Department of Life Science,undefined

[12] Cheju National University,undefined

[13] Jeju 690-756,undefined

[14] Korea,undefined

[15] Department of Environmental Engineering,undefined

[16] Cheju National University,undefined

[17] Jeju 690-756,undefined

[18] Korea,undefined

[19] School of Civil,undefined

[20] Urban & Geosystem Engineering,undefined

[21] Seoul National University,undefined

[22] Seoul 151-742,undefined

[23] Korea,undefined

[24] Biotechnology Center for Agriculture and the Environment,undefined

[25] Rutgers University,undefined

[26] New Brunswick,undefined

[27] NJ 08901-8520,undefined

[28] USA,undefined

[29] Department of Life Science,undefined

[30] Soonchunhyang University,undefined

[31] P.O. Box 97,undefined

[32] Asan,undefined

[33] Chung-Nam 336-600,undefined

[34] Korea,undefined

来源：

Applied Microbiology and Biotechnology | 2002年 / 60卷

关键词：

Enzyme; Lipid; Hydrocarbon; Amino Acid Sequence; PAHs;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Pseudomonas rhodesiae KK1 was isolated from a former manufactured-gas plant site, due to its ability to grow rapidly in a mixture of polycyclic aromatic hydrocarbons (PAHs). Radiorespirometric analysis revealed that strain KK1 was found to be able to mineralize anthracene, naphthalene and phenanthrene. Notably, phenanthrene-grown cells were able to mineralize anthracene much more rapidly than naphthalene-grown cells. Comparative analysis of amino acid sequences from 17 randomly selected dioxygenases capable of hydroxylating unactivated aromatic nuclei indicated that the enzymes for catabolism of PAHs, such as naphthalene and phenanthrene, might exist redundantly in strain KK1. Northern hybridization for cells grown on naphthalene or phenanthrene, using the putative naphthalene or phenanthrene dioxygenase gene fragment as a probe, suggested that the enzyme for naphthalene catabolism might share some homology in deduced amino acid sequences with phenanthrene dioxygenases. Also, it was found that three lipids (17:0 cyclo, 18:1 ω7c, 19:0 cyclo) increased in response to both naphthalene and phenanthrene, while the shift of other lipids varied from substrate to substrate.

引用

页码：475 / 480

页数：5