Targeted Metabolomics of Dried Blood Spot Extracts

被引：0

作者：

Sven Zukunft

Martina Sorgenfrei

Cornelia Prehn

Gabriele Möller

Jerzy Adamski

机构：

[1] Helmholtz Zentrum München,Institute of Experimental Genetics, Genome Analysis Center

[2] German Research Center for Environmental Health,undefined

[3] Technische Universität München,undefined

[4] German Center for Diabetes Research,undefined

来源：

Chromatographia | 2013年 / 76卷

关键词：

Dried blood spots; Mass spectrometry; Targeted metabolomics; Lipids; Metabolite quantification;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Dried blood spot (DBS) samples are already successfully used in newborn screening and pharmacological analyses. The application of DBS matrix to further metabolomic methods will considerably extend the analytical options for the diagnostics of metabolic diseases. We present an MS/MS based method for the simultaneous extraction and quantification of 188 metabolites from dried blood spots. We provide a sensitive and reproducible method that adapts the AbsoluteIDQ™ p180 kit of Biocrates to the DBS matrix for the quantification of metabolites of different substance classes including amino acids, biogenic amines, free carnitine, acylcarnitines, hexoses, glycerophospholipids, lysophosphatidylcholines, phosphatidylcholines, and sphingolipids.

引用

页码：1295 / 1305

页数：10

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