Targeted Metabolomics of Dried Blood Spot Extracts

被引：0

作者：

Sven Zukunft

Martina Sorgenfrei

Cornelia Prehn

Gabriele Möller

Jerzy Adamski

机构：

[1] Helmholtz Zentrum München,Institute of Experimental Genetics, Genome Analysis Center

[2] German Research Center for Environmental Health,undefined

[3] Technische Universität München,undefined

[4] German Center for Diabetes Research,undefined

来源：

Chromatographia | 2013年 / 76卷

关键词：

Dried blood spots; Mass spectrometry; Targeted metabolomics; Lipids; Metabolite quantification;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Dried blood spot (DBS) samples are already successfully used in newborn screening and pharmacological analyses. The application of DBS matrix to further metabolomic methods will considerably extend the analytical options for the diagnostics of metabolic diseases. We present an MS/MS based method for the simultaneous extraction and quantification of 188 metabolites from dried blood spots. We provide a sensitive and reproducible method that adapts the AbsoluteIDQ™ p180 kit of Biocrates to the DBS matrix for the quantification of metabolites of different substance classes including amino acids, biogenic amines, free carnitine, acylcarnitines, hexoses, glycerophospholipids, lysophosphatidylcholines, phosphatidylcholines, and sphingolipids.

引用

页码：1295 / 1305

页数：10

共 50 条

[21] Doping control analysis of trimetazidine in dried blood spot
Okano, Masato
Miyamoto, Asami
Ota, Masanori
Kageyama, Shinji
Sato, Mitsuhiko
DRUG TESTING AND ANALYSIS, 2024, 16 (08) : 766 - 776
[22] Quantification of multiple elements in dried blood spot samples
Pedersen, Lise
Andersen-Ranberg, Karen
Hollergaard, Mads
Nybo, Mads
CLINICAL BIOCHEMISTRY, 2017, 50 (12) : 703 - 709
[23] Comparison of maternal venous blood metabolomics collected as dried blood spots, dried blood microsamplers, and plasma for integrative environmental health research
Petrick, Lauren
Guan, Haibin
Page, Grier P.
Dolios, Georgia
Niedzwiecki, Megan M.
Wright, Robert O.
Wright, Rosalind J.
ENVIRONMENT INTERNATIONAL, 2024, 187
[24] Dried Blood Spot Technique Applied in Therapeutic Drug Monitoring of Anticancer Drugs: a Review on Conversion Methods to Correlate Plasma and Dried Blood Spot Concentrations
Iacuzzi, Valentina
Posocco, Bianca
Zanchetta, Martina
Gagno, Sara
Poetto, Ariana Soledad
Guardascione, Michela
Toffoli, Giuseppe
PHARMACEUTICAL RESEARCH, 2021, 38 (05) : 759 - 778
[25] Clinical perspectives of dried blood spot protein quantification using mass spectrometry methods
Lehmann, Sylvain
Picas, Alexia
Tiers, Laurent
Vialaret, Jerome
Hirtz, Christophe
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES, 2017, 54 (03) : 173 - 184
[26] Effects of Long-Term Storage on the Biobanked Neonatal Dried Blood Spot Metabolome
Ottosson, Filip
Russo, Francesco
Abrahamsson, Anna
MacSween, Nadia
Courraud, Julie
Nielsen, Zaki Krag
Hougaard, David M.
Cohen, Arieh S.
Ernst, Madeleine
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2023, 34 (04) : 685 - 694
[27] Blood sampling using "dried blood spot": a clinical biology revolution underway?
Hirtz, Christophe
Lehmann, Sylvain
ANNALES DE BIOLOGIE CLINIQUE, 2015, 73 (01) : 25 - 37
[28] Detection of autologous blood transfusions using a novel dried blood spot method
Cox, Holly D.
Miller, Geoffrey D.
Lai, Auriella
Cushman, Dan
Eichner, Daniel
DRUG TESTING AND ANALYSIS, 2017, 9 (11-12) : 1713 - 1720
[29] Interlaboratory Assessment of Dried Blood Spot Fatty Acid Compositions
Metherel, Adam H.
Harris, William S.
Ge, Liu
Gibson, Robert A.
Chouinard-Watkins, Raphael
Bazinet, Richard P.
Liu, Lei
Brenna, J. Thomas
Aristizabal-Henao, Juan J.
Stark, Ken D.
Block, Robert C.
LIPIDS, 2019, 54 (11-12) : 755 - 761
[30] Dried blood spot sampling of testosterone microdosing in healthy females
Desai, Reena
Savkovic, Sasha
Handelsman, David J.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2024, 240

← 1 2 3 4 5 →