The effect of brain-derived neurotrophic factor on angiogenesis

被引：0
作者：
Chunyan Sun
Yu Hu
Zhangbo Chu
Jing Huang
Lu Zhang
机构：
[1] Huazhong University of Science and Technology,Institute of Hematology, Union Hospital, Tongji Medical College
来源：
Journal of Huazhong University of Science and Technology [Medical Sciences] | 2009年 / 29卷
关键词：
brain-derived neurotrophic factor; angiogenesis; human umbilical vein endothelial cells;
D O I：
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学科分类号：
摘要：
To investigate the in vitro and in vivo proangiogenic effects of brain-derived neurotrophic factor (BDNF), human umbilical vein endothelial cells (HUVECs) were isolated and cultured in primary culture. The effect of BDNF on the proliferation of HUVECs was examined by MTT assay. The effects of BDNF on HUVEC migration and tube formation were studied by modified Boyden chamber assay and tube formation assay, respectively. Matrigel plug assay and chorioallantoic membrane assay were used to evaluate the effects of BDNF on angiogenesis in vivo. Our results showed that BDNF substantially stimulated the migration and tube formation of HUVECs in vitro, although it did not induce HUVEC proliferation. BDNF also induced angiogenesis both in matrigel plug of mouse model and in chick chorioallantoic membrane. In conclusion, BDNF can promote angiogenesis both in vitro and in vivo, and may be a proangiogenic factor.
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页码：139 / 143
页数：4
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