Cryo-Electron Microscopy at the Biological Faculty of Moscow State University

Abstract: The paper reports an example of a successful upgrade of the JEOL JEM-2100 analytical transmission electron microscope to a low-resolution cryo-electron microscope, which can be used to optimize sample preparation and to assess preparation quality. As a result of the instrument upgrade, it is possible to obtain subnanometer resolution of protein molecule reconstructions (within 8 Å). The role of graphene and amorphous carbon substrates in preventing the effect of preferential orientation of protein particles in a frozen sample is discussed. © Allerton Press, Inc. 2023. ISSN 0096-3925, Moscow University Biological Sciences Bulletin, 2023, Vol. 78, Suppl. 1, pp. S59–S63. Allerton Press, Inc., 2023. Russian Text The Author(s), 2023, published in Vestnik Moskovskogo Universiteta, Seriya 16: Biologiya, 2023, Vol. 78, No. 3 Supplement, pp. 51–56.