Physicochemical characterization and antioxidant activity of a polysaccharide isolated from oolong tea

被引：0
作者：
Haixia Chen
Zhaoshuai Wang
Zhishuang Qu
Lingling Fu
Peng Dong
Xin Zhang
机构：
[1] Tianjin University,Tianjin Key Laboratory for Modern Drug Delivery and High
来源：
European Food Research and Technology | 2009年 / 229卷
关键词：
Oolong tea polysaccharide; Composition; Antioxidant activity; Atomic force microscopy; Laser light scattering analysis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Oolong tea polysaccharide (OTPS) is a kind of water-soluble polysaccharide isolated from oolong tea. Its molecular weight, composition, and structure were analyzed by laser light scattering analysis, chemical methods, gas chromatography, and atomic force microscopy (AFM). Antioxidant potential was detected by hydroxyl radical scavenging, inhibition of lipid peroxidation, DPPH radical scavenging, and reducing power assays. Results showed that OTPS was a non-reductive acid heteropolysaccharide bound to protein. The amounts of uronic acid, protein and neutral sugar in OTPS were 40.65, 19.59, and 26.66%, respectively. The neutral sugar was composed of d-rhamnose, l-arabinose, d-galactose, and d-glucose with a molar ratio of 1.37:1.89:1.00:1.30. The molecular weight of OTPS was 1.28 × 106 Da and the second viral coefficient was 2.36 × 10−4 cm3 mol/g2. A globular image of OTPS was imaged by AFM. OTPS showed effective antioxidant activities on hydroxyl radical scavenging assay and inhibition assay of lipid peroxidation.
引用
收藏
页码：629 / 635
页数：6
相关论文
共 99 条
  • [1] Lin JK(1998)undefined J Agric Food Chem 46 3635-3642
  • [2] Lin CL(2008)undefined Metab Clin Exp 57 526-534
  • [3] Liang YC(1999)undefined Int J Obes Relat Metab Disord 23 98-105
  • [4] Lin-Shiau SY(2004)undefined J Agric Food Chem 52 3333-3336
  • [5] Juan IM(2008)undefined Food Chem 106 559-563
  • [6] Tanida M(2001)undefined J Agric Food Chem 49 507-510
  • [7] Tsuruoka N(2008)undefined Food Chem 111 428-432
  • [8] Shen J(2006)undefined Carbohydr Res 341 2261-2269
  • [9] Kiso Y(2005)undefined Int J Biol Macromol 37 195-199
  • [10] Nagai K(2007)undefined Carbohydr Polym 67 213-218
12345678910