Characterization of Cellobiohydrolases from Schizophyllum commune KMJ820

被引：0

作者：

Sanath Kondaveeti

Sanjay K. S. Patel

Janghun Woo

Ji Hyang Wee

Sang-Yong Kim

Riyadh I. Al-Raoush

In-Won Kim

Vipin Chandra Kalia

Jung-Kul Lee

机构：

[1] Konkuk University,Department of Chemical Engineering

[2] Shin-Ansan University,Department of Food Science and Biotechnology

[3] Qatar University,Department of Civil and Architectural Engineering, College of Engineering

来源：

Indian Journal of Microbiology | 2020年 / 60卷

关键词：

Cellobiohydrolase; Catalytic efficiency; Enzyme purification; Enzyme production;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as Schizophyllum commune KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using S. commune supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity (Vmax = 51.4 and 20.8 U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of S. commune CBH 1 and 2 were verified to be approximately 50 kDa and 150 kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but S. commune CBH is prominent in comparison to other CBHs by its high enzyme activity.

引用

页码：160 / 166

页数：6