Characterization of siRNAs derived from cucumber mosaic virus in infected tobacco plants

被引：0
作者：
Yanhong Qiu
Yongjiang Zhang
Fan Hu
Shuifang Zhu
机构：
[1] Chinese Academy of Inspection and Quarantine,Institute of Plant Quarantine
[2] China Agricultural University,undefined
来源：
Archives of Virology | 2017年 / 162卷
关键词：
Cucumber Mosaic Virus; Rice Stripe Virus; Cucumber Green Mottle Mosaic Virus; Viral Suppressor; Cucumber Mosaic Virus Infection;
D O I：
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学科分类号：
摘要：
This study characterized the viral small interfering RNAs (vsiRNAs) from Nicotiana tabacum cv. Samsun infected with a cucumber mosaic virus (CMV) 2b-deficient mutant. Most vsiRNAs were 21 -22 nucleotides in length and the 5′-terminal ends were dominated by A and U, respectively. The observed vsiRNAs were heterogeneously distributed throughout the CMV genome; however, most of the vsiRNAs were derived from sense strands, as opposed to antisense strands. These results demonstrate the conserved and specific function of Dicer-like (DCL), Argonaute (AGO) and RNA-dependent RNA polymerase (RDR) proteins in tobacco. Finally, it was revealed that vsiRNAs target abundant host genes, indicating complex roles for CMV vsiRNAs during the development of symptoms.
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页码：2077 / 2082
页数：5
相关论文
共 202 条
[1]  
Wang MB(2012)RNA silencing and plant viral diseases Mol Plant Microbe Interact 25 1275-1285
[2]  
Masuta C(2007)Antiviral immunity directed by small RNAs Cell 130 413-426
[3]  
Smith NA(2004)RNA silencing in plants Nature 431 356-363
[4]  
Shimura H(2013)Plant RNA interference pathways: diversity in function, similarity in action Plant Mol Biol Rep 31 493-506
[5]  
Ding SW(2010)RNA-based antiviral immunity Nat Rev Immunol 10 632-644
[6]  
Voinnet O(2010)RNAi-mediated viral immunity requires amplification of virus-derived siRNAs in PNAS 107 484-489
[7]  
Baulcombe D(2013)Recent Advances in plant-virus interaction with emphasis on small interfering RNAs (siRNAs) Mol Biotechnol 55 63-77
[8]  
Pattanayak D(2013)RNA silencing suppression by plant pathogens: defence, counter—defence and counter-counter-defence Nat Rev Microbiol 11 745-760
[9]  
Solanke AU(2011)Viral suppressors of RNA silencing Trends Plant Sci 16 265-272
[10]  
Kumar PA(2011)Viral small interfering RNAs target host genes to mediate disease symptoms in plants PloS Pathog 7 e1002022-1119
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