Utility of barley and wheat simple sequence repeat (SSR) markers for genetic analysis of Hordeum chilense and tritordeum

被引:0
作者
P. Hernández
D. A. Laurie
A. Martín
J. W. Snape
机构
[1] Instituto de Agricultura Sostenible (CSIC),
[2] Apartado 4084,undefined
[3] 14080 Cordoba,undefined
[4] Spain e-mail: ge1hemop@uco.es Tel.: +34-957-499210,undefined
[5] Fax: +34-957-499252,undefined
[6] John Innes Centre,undefined
[7] Norwich Research Park,undefined
[8] Colney,undefined
[9] Norwich NR4 7UH,undefined
[10] UK,undefined
来源
Theoretical and Applied Genetics | 2002年 / 104卷
关键词
Keywords  Hordeum chilense; Tritordeum; Introgression; Marker-assisted selection; Mapping; Microsatellite; SSR;
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摘要
A selection of 36 wheat and 35 barley simple sequence repeat markers (SSRs) were studied for their utility in Hordeum chilense. Nineteen wheat and nineteen barley primer pairs amplified consistent H. chilense products. Nine wheat and two barley SSRs were polymorphic in a H. chilense mapping population, producing codominant markers that mapped to the expected homoeologous linkage groups in all but one case. Thirteen wheat and 10 barley primer pairs were suitable for studying the introgression of H. chilense into wheat because they amplified H. chilense products of distinct size. Analysis of wheat/H. chilense addition lines showed that the H. chilense products derived from the expected homoeologous linkage groups. The results showed that wheat and barley SSRs provide a valuable resource for the genetic characterization of H. chilense, tritordeums and derived introgression lines.
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页码:735 / 739
页数:4
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