A new primer for 16S rDNA analysis of microbial communities associated with Porphyra yezoensis

被引：0
|
作者：
Aki Namba
Yuya Shigenobu
Masahiro Kobayashi
Takanori Kobayashi
Ichiro Oohara
机构：
[1] Fisheries Research Agency,Aquatic Genomics Research Center, National Research Institute of Fisheries Science
[2] Fisheries Research Agency,Research Center for Fisheries and Environment in the Ariake and Yatsushiro Bays, Seikai National Fisheries Research Institute
[3] Nihon University,Laboratory of Fish Pathology, Department of Veterinary Medicine
来源：
Fisheries Science | 2010年 / 76卷
关键词：
16S rDNA; Polymerase chain reaction; Universal primer; Microbial community;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
To construct high-quality 16S rDNA clone libraries for microbial communities associated with Porphyra yezoensis and to minimize the detection of rDNA from leafy gametophytes of P. yezoensis, we designed a new 16S rDNA universal primer (75F). Of the clones prepared using 75F, which was designed to distinguish between bacteria and P. yezoensis, 95% were classified into four groups, namely, β-proteobacteria, γ-proteobacteria, Lentisphaerae, and Flavobacteria. PCR-based analysis of the 16S rDNA primer constructed in this study can be used to implement 16S rDNA-based methodologies for the investigation of microbial community composition and diversity related to the Porphyra group.
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页码：873 / 878
页数：5
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