Identification of false-positive CBFβ/MYH11 RT-PCR results

被引：0
|
作者：
SM Hackwell
DO Robinson
JF Harvey
FM Ross
机构：
[1] Wessex Regional Genetics Laboratory,
[2] Salisbury District Hospital,undefined
来源：
Leukemia | 1999年 / 13卷
关键词：
acute myeloid leukaemia; CBFβ/MYH11 transcripts; RT-PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Persistent problems with false positive results were encountered when carrying out a published RT-PCR method to detect the CBFβ/MYH11 transcripts associated with the inv(16)(p13q22) cytogenetic abnormality in acute myeloid leukaemia. These were shown to be due to amplification of part of the intronic MYH11 sequence, presumably from very small amounts of contaminating DNA or unspliced primary RNA transcripts, amplified because of partial homology of the CBFβ3 primer to intronic MYH11 sequence.
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页码：1617 / 1619
页数：2
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