Proteolytic Activity from an Alkali-Thermotolerant Streptomyces gulbargensis sp. nov.

被引：0
作者：
S. G. Dastager
Agasar Dayanand
Wen-Jun Li
Chang-Jin Kim
Jae-Chan Lee
Dong-Jin Park
Xin-Peng Tian
Q. S. Raziuddin
机构：
[1] Korea Research Institute of Bioscience and Biotechnology,Laboratory for Conservation and Utilization of Bio
[2] Yunnan University,Resources, Yunnan Institute of Microbiology
[3] Gulbarga University,Department of Studies and Research in Microbiology
[4] Chinese Academy of Sciences,Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology
来源：
Current Microbiology | 2008年 / 57卷
关键词：
Streptomyces; Alkaline Protease; Protease Production; Nocardiopsis; Skim Milk Agar;
D O I：
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中图分类号：
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摘要：
Multiple proteases were produced and partially purified from an alkali-thermotolerant novel species of Streptomyces (i.e., Streptomyces gulbargensis DAS 131) after 48 h of growth at 45°C. The enzyme preparation exhibited activity over a broad range of pH (4–12) and temperature (27–55°C). Optimum activity was observed at a pH of 9.0 and a temperature of 45°C. Starch and protease peptone was found to be a good source of carbon and nitrogen to enhance the enzyme activity. Two active zones in the range of 19 to 35 kDa were detected on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
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[11]  
Cui XL(1995)Production of a thermophilic, extracellular alkaline protease by J Appl Bacteriol 78 378-383
[12]  
Mao PH(2004) AP-4 Int J Syst Evol Microbiol 54 247-252
[13]  
Tseng M(1991)Comparative characterization of two serine endopeptidases from Curr Microbiol 22 377-382
[14]  
Li WJ(1995) sp. NCIM 5124 Electrophoresis 16 43-45
[15]  
Zhang LP(2002)Bioactive rare actinomycetes from indigenous natural substrates of Andhra Pradesh Biotechnol Biochem 66 164-167
[16]  
Xu LH(1991)Chitinase production by Agric Biol Chem 55 307-314
[17]  
Jiang CL(1979): its potential on fungal cell wall lysis Eur J Appl Microbiol Biotechnol 9 199-209
[18]  
Daniel RM(1998) sp. nov.: a novel alkaliphilic actinomycetes isolated from desert soil in Egypt Microbiol Mol Biol Rev 62 597-635
[19]  
Toogood HS(1989)Extracellular protease activity in antibiotic-producing Appl Microbiol Biotechnol 31 38-44
[20]  
Bergquist PL(2002)A new method for the detection of proteolytic activity in Int J Syst Evol Microbiol 52 2291-2295
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