Proteolytic Activity from an Alkali-Thermotolerant Streptomyces gulbargensis sp. nov.

被引：0
作者：
S. G. Dastager
Agasar Dayanand
Wen-Jun Li
Chang-Jin Kim
Jae-Chan Lee
Dong-Jin Park
Xin-Peng Tian
Q. S. Raziuddin
机构：
[1] Korea Research Institute of Bioscience and Biotechnology,Laboratory for Conservation and Utilization of Bio
[2] Yunnan University,Resources, Yunnan Institute of Microbiology
[3] Gulbarga University,Department of Studies and Research in Microbiology
[4] Chinese Academy of Sciences,Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology
来源：
Current Microbiology | 2008年 / 57卷
关键词：
Streptomyces; Alkaline Protease; Protease Production; Nocardiopsis; Skim Milk Agar;
D O I：
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摘要：
Multiple proteases were produced and partially purified from an alkali-thermotolerant novel species of Streptomyces (i.e., Streptomyces gulbargensis DAS 131) after 48 h of growth at 45°C. The enzyme preparation exhibited activity over a broad range of pH (4–12) and temperature (27–55°C). Optimum activity was observed at a pH of 9.0 and a temperature of 45°C. Starch and protease peptone was found to be a good source of carbon and nitrogen to enhance the enzyme activity. Two active zones in the range of 19 to 35 kDa were detected on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
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[1]  
Bakhtiar S(2003)Crystallization and preliminary X-ray analysis of an alkaline serine protease from Acta Crystallgr D Biol Crystallogr 59 529-531
[2]  
Vevodova J(1983) sp J Ferment Technol 61 511-514
[3]  
Hatti-Kaul R(1987)A low-cost method for the production of extracellular alkaline proteinase using tapioca starch Arch Biochem Biophys 257 395-401
[4]  
Su XD(1998)Multiple protease from J Bichem Biophys 35 34-40
[5]  
Chandarsekarn S(2001) I. Isolation and purification of five extracellular proteases Int J Syst Evol Microbiol 51 357-363
[6]  
Dhar SC(1995)Thermostable alkaline mettalloprotease from newly isolated alkaliphilic Biotech Genet Eng Rev 13 51-100
[7]  
Chandarsekarn S(2007) strain SSI Antonie Van Leeuwenhoek 9 99-104
[8]  
Dhar SC(1994) gen.vov., a new member of the family World J Microbiol Biotechnol 10 33-35
[9]  
Chaphalkar SR(2000)Thermostable proteases Biochimica et Biophysica Acta 1523 261-268
[10]  
Dey S(2002) sp.nov. isolated from soil in Karnataka, India Hindustan Antibiot Bull 44 17-24
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