The synthesis of P1-[11-(anthracen-9-ylmethoxy)undecyl]-P2-(2-acetamido-2-deoxy-α-D-glucopyranosyl) diphosphate and the study of its acceptor properties in the enzymatic reaction catalyzed by a D-rhamnosyltransferase from Pseudomonas aeruginosa

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作者
A. N. Vinnikova
V. I. Torgov
N. S. Utkina
V. V. Veselovsky
T. N. Druzhinina
S. Wang
I. Brockhausen
L. L. Danilov
机构
[1] Russian Academy of Sciences,Zelinsky Institute of Organic Chemistry
[2] Queen’s University,Department of Medicine, and Department of Biomedical and Molecular Sciences
关键词
P; -[11-(anthracen-9-ylmethoxy)undecyl]-P; -(2-acetamido-2-deoxy-α-; -glucopyranosyl) diphosphate; fluorescent substrate; -rhamnosyltransferase; Pseudomonas aeruginosa PAO1;
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摘要
P1-[11-(anthracen-9-ylmethoxy)undecyl]-P2-(2-acetamido-2-deoxy-α-D-glucopyranosyl) diphosphate, a fluorescent derivative of undecyl diphosphate 2-acetamido-2-deoxyglucose, was chemically synthesized. The ability of the compound to serve as acceptor substrate for the transfer of D-rhamnose residue by D-rhamnosyltransferase WbpZ from Pseudomonas aeruginosa PAO1 was demonstrated.
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页码:105 / 107
页数:2
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