Identification of European mosquito species by MALDI-TOF MS

被引：0
作者：
Amina Yssouf
Philippe Parola
Anders Lindström
Tobias Lilja
Grégorie L’Ambert
Ulf Bondesson
Jean-Michel Berenger
Didier Raoult
Lionel Almeras
机构：
[1] Aix Marseille Université,Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63, CNRS 7278, IRD 198 (Dakar, Sénégal), Inserm 1095, WHO Collaborative Center for Rickettsioses and Other Arthropod
[2] National Veterinary Institute (SVA),Borne Bacterial Diseases, Faculté de Médecine
[3] Entente Interdépartementale de Démoustication Méditerranée,Department of Chemistry, Environment and Feed Hygiene
来源：
Parasitology Research | 2014年 / 113卷
关键词：
MALDI-TOF mass spectrometry; Mosquito; Identification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
MALDI-TOF MS profiling has proved to be efficient for arthropod identification at the species level. However, prior to entomological monitoring, the reference spectra database should cover relevant species. Here, 74 specimens were field-collected from 11 mosquito species captured in two distinct European areas and used either to increment our database or for blind tests. Misidentification was not noted, underlining the power of this approach. Nevertheless, three out of the 26 specimens used for the blind test did not reach the significant identification threshold value set, attributed to lower spectral quality. In the future, the quality control spectra parameters need to be defined to avoid not achieving significant threshold identification.
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页码：2375 / 2378
页数：3
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