Tas1r3, encoding a new candidate taste receptor, is allelic to the sweet responsiveness locus Sac

被引：162

作者：

Max M. ^{[1
]}

Shanker Y.G. ^{[1
,2
]}

Huang L. ^{[1
,2
]}

Rong M. ^{[1
,2
]}

Liu Z. ^{[1
,2
]}

Campagne F. ^{[1
,3
]}

Weinstein H. ^{[1
,3
]}

Damak S. ^{[1
]}

Margolskee R.F. ^{[1
,2
]}

机构：

[1] Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York University, New York, NY

[2] Howard Hughes Medical Institute, Mount Sinai School of Medicine, New York University, New York, NY

[3] Institute for Computational Biomedicine, Mount Sinai School of Medicine, New York University, New York, NY

来源：

Nature Genetics | 2001年 / 28卷 / 1期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/ng0501-58

中图分类号：

学科分类号：

摘要：

The ability to taste the sweetness of carbohydrate-rich foodstuffs has a critical role in the nutritional status of humans. Although several components of bitter transduction pathways have been identified1-6, the receptors and other sweet transduction elements remain unknown. The Sac locus in mouse, mapped to the distal end of chromosome 4 (refs. 7-9), is the major determinant of differences between sweet-sensitive and -insensitive strains of mice in their responsiveness to saccharin, sucrose and other sweeteners10-13. To identify the human Sac locus, we searched for candidate genes within a region of approximately one million base pairs of the sequenced human genome syntenous to the region of Sac in mouse. From this search, we identified a likely candidate: T1R3, a previously unknown G protein-coupled receptor (GPCR) and the only GPCR in this region. Mouse Tas1r3 (encoding T1r3) maps to within 20,000 bp of the marker closest to Sac (ref. 9) and, like human TAS1R3, is expressed selectively in taste receptor cells. By comparing the sequence of Tas1r3 from several independently derived strains of mice, we identified a specific polymorphism that assorts between taster and non-taster strains. According to models of its structure, T1r3 from non-tasters is predicted to have an extra amino-terminal glycosylation site that, if used, would interfere with dimerization.

引用

页码：58 / 63

页数：5

共 25 条

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