Genetic Stability of Human Endometrial Mesenchymal Stem Cells Assessed with Morphological and Molecular Karyotyping

被引：2

作者：

Shilina M.A. ^{[1
]}

Grinchuk T.M. ^{[1
]}

Nikolsky N.N. ^{[1
]}

机构：

[1] Institute of Cytology, Russian Academy of Sciences, St. Petersburg

来源：

Cell and Tissue Biology | 2017年 / 11卷 / 1期

关键词：

karyotyping; molecular karyotyping; stem cells;

D O I：

10.1134/S1990519X17010114

中图分类号：

学科分类号：

摘要：

The aim of this study was to monitor the genetic stability of endometrial mesenchymal stem cells (eMSCs) by G-banding and molecular karyotyping. We evaluated the sensitivity of each method to assess the genetic stability of eMSCs. G-banding karyotyping performed on passages 6 and 15 showed that more than 80% cells had normal karyotype. Random karyotypic changes were found in a small part of the cell population: aneuploidy, isochromosomes, chromosome breakages, interchromosomal association. Molecular karyotyping carried out on the 6th and 14th passages revealed genomic stability, except for in the case of chromosomes 7 and 14. Microduplications 7q36.3 (62 kb) and 14q11.2 (165kb) were found in these chromosomes. We interpreted these aberrations as being derived from the donor of these cells. The morphological and molecular karyotyping complemented each other. Using these methods, we can analyze karyotypic stability at different levels of the genomic organization. © 2017, Pleiades Publishing, Ltd. All Right Reserved.

引用

页码：35 / 41

页数：6

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