TGF-β1-siRNA delivery with nanoparticles inhibits peritoneal fibrosis

被引：0
作者：
H Yoshizawa
Y Morishita
M Watanabe
K Ishibashi
S Muto
E Kusano
D Nagata
机构：
[1] Jichi Medical University,Division of Nephrology, Department of Internal Medicine
[2] Meiji Pharmaceutical University,Department of Medical Physiology
来源：
Gene Therapy | 2015年 / 22卷
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摘要：
Gene therapies may be promising for the treatment of peritoneal fibrosis (PF) in subjects undergoing peritoneal dialysis (PD). However, a method of delivery of treatment genes to the peritoneum is lacking. We attempted to develop an in vivo small interfering RNA (siRNA) delivery system with liposome-based nanoparticles (NPs) to the peritoneum to inhibit PF. Transforming growth factor (TGF)-β1-siRNAs encapsulated in NPs (TGF-β1-siRNAs-NPs) dissolved in PD fluid were injected into the peritoneum of mice with PF three times a week for 2 weeks. TGF-β1-siRNAs-NPs knocked down TGF-β1 expression significantly in the peritoneum and inhibited peritoneal thickening with fibrous changes. TGF-β1-siRNAs-NPs also inhibited the increase of expression of α-smooth muscle actin-positive myofibroblasts. These results suggest that the TGF-β1-siRNA delivery system with NPs described here could be an effective therapeutic option for PF in subjects undergoing PD.
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页码：333 / 340
页数：7
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