In vivo distribution of receptor for ecotropic murine leukemia virus and binding of envelope protein of Friend Murine leukemia virus

被引：0

作者：

S. Yamaguchi

M. Hasegawa

T. Suzuki

H. Ikeda

S. Aizawa

K. Hirokawa

M. Kitagawa

机构：

[1] Department of Pathology and Immunology,

[2] Aging and Developmental Sciences,undefined

[3] Tokyo Medical and Dental University,undefined

[4] Graduate School,undefined

[5] Tokyo,undefined

[6] Japan,undefined

[7] Radiation Hazards Research Group,undefined

[8] National Institute of Radiological Sciences,undefined

[9] Chiba,undefined

[10] Japan,undefined

[11] Laboratory of Immunogenetics,undefined

[12] National Institute of Animal Health,undefined

[13] Tsukuba,undefined

[14] Ibaraki,undefined

[15] Japan,undefined

[16] Laboratory of Infectious Diseases,undefined

[17] National Institute of Animal Health,undefined

[18] Tsukuba,undefined

[19] Ibaraki,undefined

[20] Japan,undefined

来源：

Archives of Virology | 2003年 / 148卷

关键词：

mRNA Expression; Green Fluorescence Protein; Binding Assay; Interference Effect; Envelope Protein;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Ecotropic infection by Murine leukemia virus (MuLV) infection is initiated by the interaction between the receptor-binding domain of the viral surface glycoprotein (SU) and the cell-surface receptor, mCAT-1. To study the in vivo localization of viral binding site in mice, green fluorescence protein (GFP)-tagged Friend SU (F-SU/GFP) was incubated with tissue sections. Lymphohematopoietic organs and a part of the glandular tissues of C3H as well as C57BL/6 mice revealed positive signals for F-SU/GFP binding on the cell surface. In contrast, C4W mice, which is a partial congenic mouse strain carrying the Fv-4r gene on a BALB/c genetic background, exhibited negative signals in most of the organs except for a very weak binding in the pancreas. The expression of mCAT-1 mRNA determined by reverse transcriptase (RT)-polymerase chain reaction (PCR) revealed a similar distribution in C3H, C57BL/6 and C4W mice. Most of the organs including lymphohematopoietic organs and glandular organs revealed significant expression of mRNA for mCAT-1 gene, while the liver, heart and muscle did not. The results from binding assay were consistent with the fact that Friend MuLV-induced pathogenesis was usually associated with lymphohematopoietic systems, although mRNA expression for mCAT-1 was rather ubiquitous. The discrepancy between F-SU/GFP binding and mRNA expression for mCAT-1 in lymphohematopoietic organs of C4W mice would support the receptor interference effect by the Fv-4r gene causing the resistance of C4W mouse to Friend MuLV infection.

引用

页码：1175 / 1184

页数：9