Development and characterization of 10 polymorphic microsatellite loci for the blue shark, Prionace glauca, and their cross shark-species amplification

被引：0
作者：
Seán Fitzpatrick
Mahmood S. Shivji
Demian D. Chapman
Paulo A. Prodöhl
机构：
[1] Medical Biology Centre,School of Biological Sciences
[2] Queen’s University Belfast,School of Marine and Atmospheric Science
[3] Guy Harvey Research Institute and Save Our Seas Shark Centre,undefined
[4] Oceanographic Centre,undefined
[5] Nova South-Eastern University,undefined
[6] Institute for Ocean Conservation Science,undefined
[7] Stony Brook University,undefined
来源：
Conservation Genetics Resources | 2011年 / 3卷
关键词：
Blue shark; Polymorphic; Microsatellite; Cross-species;
D O I：
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中图分类号：
学科分类号：
摘要：
Ten polymorphic nuclear microsatellite loci were developed from a microsatellite enriched genomic library of the blue shark, Prionace glauca. The utility of these markers for genetic studies of this globally distributed, heavily exploited, oceanic predator was assessed by screening 120 specimens sampled from six locations throughout the species’ range. Both moderately and highly polymorphic marker loci were identified. Three to 35 alleles were found to be segregating per locus (mean 10.1) with observed heterozygosities ranging from 24 to 91%. Evaluation of the cross-species amplification of these markers across 18 additional shark species indicates that these microsatellites are potentially useful for genetic studies of other species of conservation concern.
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页码：523 / 527
页数：4
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  • [1] Boston E(2009)Development and characterization of 11 polymorphic compound tetranucleotide microsatellite loci for the Leisler’s bat, Conserv Genet 10 1501-1504
  • [2] Montgomery WI(2004) (Vespertilionidae, Chiroptera) Mol Ecol 13 1965-1974
  • [3] Prodöhl PA(2007)Predominance of genetic monogamy by females in a hammerhead shark, Biol Lett 3 425-427
  • [4] Chapman DD(2006): implications for shark conservation Conserv Biol 20 201-211
  • [5] Prodöhl PA(2008)Virgin birth in a hammerhead shark Mol Ecol 17 783-795
  • [6] Gelsleichter J(2010)Identification of shark species composition and proportion in the Hong Kong shark fin market based on molecular genetics and trade records J Hered 101 374-377
  • [7] Manire CA(1999)Are indirect genetic benefits associated with polyandry? Testing predictions in a natural population of lemon sharks Copeia 1999 182-186
  • [8] Shivji MS(1994)Shark virgin birth produces multiple, viable offspring BioTech 16 657-662
  • [9] Chapman DD(2006)Microsatellite DNA variation in sandbar sharks (Carcharhinus plumbeus) from the Gulf of Mexico and mid-Atlantic Bight J Ichthyol 46 613-624
  • [10] Shivji MS(2009)Enrichment of microsatellites from citrus genome using Biotinylated Oligonucleotide sequences bound to Streptavidin-Coated magnetic particles Conserv Genet 10 1989-1992
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