Somatic hybrids between transgenic Solanum tuberosum potato plants and transplastome Solanum rickii plants

被引：0
作者：
N. A. Matveeva
A. M. Shakhovskii
N. V. Kuchuk
机构：
[1] National Academy of Sciences of Ukraine,Institute of Cellular Biology and Genetic Engineering
来源：
Cytology and Genetics | 2008年 / 42卷
关键词：
Green Plant; Somatic Hybrid; Hybrid Plant; Spectinomycin; Fusion Product;
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摘要：
Experiments on fusion of mesophyllic protoplasts of Solanum tuberosum (Lugovskoi and Slavyanka cultivars) possessing the nptII gene in the nuclear DNA with transplastome Solanum rickii plants (which possess the aadA gene) that we have derived previously, are performed. Hybrid plants with the genes aadA and nptII, the chloroplasts of S. rickii and S. tuberosum, and a hybrid nuclear genome were obtained in a selection medium containing the antibiotics kanamycin, spectomycin, and streptomycin. The result is confirmed by results of PCR analyses.
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