A light-inducible Myb-like gene that is specifically expressed in red Perilla frutescens and presumably acts as a determining factor of the anthocyanin forma

被引:19
作者
Gong Z.-Z. [1 ]
Yamazaki M. [1 ]
Saito K. [1 ]
机构
[1] Lab. of Molec. Biol./Biotechnology, Res. Center of Medicinal Resources, Chiba University, Chiba 263-8522, Yayoi-cho 1-33, Inage-ku
来源
Molecular and General Genetics MGG | 1999年 / 262卷 / 1期
基金
日本学术振兴会;
关键词
Anthocyanin biosynthesis; Light induction MYB transcriptional factor; Perilla frutescens;
D O I
10.1007/PL00008639
中图分类号
学科分类号
摘要
The Myb-p1 gene was isolated by screening for differentially expressed Myb-related genes in red (anthocyanin-producing) and green (anthocyanin non-producing) forms of Perilla frutescens. Expression of Myb-p1 is increased 10-fold in the red relative to the green form of P. frutescens, and the gene is induced by light. MYB-P1 has only one DNA-binding region, which corresponds to repeat III in the general structure of MYB proteins. In the yeast two-hybrid system, it was shown that MYB-P1 interacted with MYC-RP, a MYC-related transcriptional regulatory protein involved in the control of anthocyanin biosynthesis in P. frutescens. In yeast, MYB-P1 was able to bind to a dihydroflavonol reductase (DFR) gene promoter isolated from red P. frutescens. These data suggest that Myb-p1 may be involved in the regulation of anthocyanin biosynthesis and could therefore be responsible for determining anthocyanin formation in red P. frutescens.
引用
收藏
页码:65 / 72
页数:7
相关论文
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