Hydroxyurea induces a senescence-like change of K562 human erythroleukemia cell

被引：0

作者：

Joo-In Park

Jin-Sook Jeong

Jin-Yeong Han

Deok-In Kim

Yan-Hua Gao

Sang-Chul Park

Griffin P. Rodgers

In-Hoo Kim

机构：

[1] Department of Biochemistry,

[2] Dong-A University College of Medicine,undefined

[3] Pusan,undefined

[4] Korea,undefined

[5] Department of Pathology,undefined

[6] Dong-A University College of Medicine,undefined

[7] Pusan,undefined

[8] Korea,undefined

[9] Department of Clinical Pathology,undefined

[10] Dong-A University College of Medicine,undefined

[11] Pusan,undefined

[12] Korea,undefined

[13] WHO Collaborating Centre of Physical Culture and Aging Research for Health Promotion,undefined

[14] Seoul National University College of Medicine,undefined

[15] Seoul,undefined

[16] Korea,undefined

[17] Molecular and Clinical Hematology Branch,undefined

[18] NIDDK/NIH,undefined

[19] Bethesda,undefined

[20] Maryland,undefined

[21] USA,undefined

来源：

Journal of Cancer Research and Clinical Oncology | 2000年 / 126卷 / 8期

关键词：

Key words Hydroxyurea; Senescence; Erythroleukemia K562 cells;

D O I：

10.1007/PL00021281

中图分类号：

学科分类号：

摘要：

Hydroxyurea, a differentiation-inducing agent of human erythroleukemia K562 cells, is commonly used to treat some types of leukemia. However, the mechanism for its therapeutic effect is not clearly understood yet. In this study, we have observed an interesting effect of hydroxyurea on tumor cells: an induction of senescence-like changes. Human erythroleukemia K562 cells, when treated with hydroxyurea for 7 days or more, underwent a change into phenotypically senescent cells together with a reduction of hemoglobin generation, a differentiation marker. The hydroxyurea-treated cells showed positive senescence associated-β-galactosidase staining, a senescence index, and the accumulation of cdk (cyclin dependent kinase) inhibitors, such as p16INK4a, p21Waf1, and p27Kip1, implicated in cellular senescence. Nonetheless, these changes were not accompanied by DNA fragmentation. Taken together, we summarize that the long-term treatment of cancer cells with hydroxyurea can induce cellular senescence different from differentiation or programmed cell death.

引用

页码：455 / 460

页数：5