An increased fraction of circulating miR-363 and miR-16 is particle bound in patients with chronic lymphocytic leukaemia as compared to normal subjects

被引：3

作者：

Alharthi A. ^{[1
]}

Beck D. ^{[1
]}

Howard D.R. ^{[2
]}

Hillmen P. ^{[2
]}

Oates M. ^{[3
]}

Pettitt A. ^{[3
]}

Wagner S.D. ^{[1
]}

机构：

[1] Leicester Cancer Research Centre, Ernest and Helen Scott Haematological Research Unit, University of Leicester, Hodgkin Building, Lancaster Road, Leicester

[2] St James Institute of Oncology, Leeds

[3] University of Liverpool, Duncan Building, Daulby Street, Liverpool

来源：

BMC Research Notes | / 11卷 / 1期

关键词：

Chronic lymphocytic leukaemia; Extracellular vesicle; miRNA;

D O I：

10.1186/s13104-018-3391-9

中图分类号：

学科分类号：

摘要：

Objectives: In vitro culture studies have shown that miR-363 is enriched in extracellular vesicles from chronic lymphocytic leukaemia cells. We wondered whether miR-363 was detectable in plasma, which is an essential precondition for further studies to assess its usefulness as a biomarker. Using samples from two clinical trials: one enrolling patients with advanced disease and the other asymptomatic patients with early stage disease, we determined plasma miR-363 levels and secondly investigated the distribution of this miRNA between plasma and particle bound fractions in patients and normal subjects. Results: Advanced disease (n = 95) was associated with higher levels of miR-363 than early stage disease (n = 45) or normal subjects (n = 11) but there was no association with markers of prognosis. The distribution of specific miRNA between particle bound and plasma protein fractions was investigated using size exclusion chromatography on plasma from patients (n = 4) and normal subjects (n = 3). ~ 20% of total miR-16 and miR-363 is particle bound in patients while there was no detectable particle bound material in normal subjects. Our work demonstrates that miR-363 levels are raised in chronic lymphocytic leukaemia patients and raises the possibility that distribution of circulating miRNA between plasma fractions differs in health and disease. © 2018 The Author(s).

引用

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