Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China

被引:0
作者
Jianfei Chen
Xiaozhen Liu
Hongwu Lang
Zhongtian Wang
Da Shi
Hongyan Shi
Xin Zhang
Li Feng
机构
[1] Chinese Academy of Agricultural Sciences,Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute
[2] China Institute of Veterinary Drug Control,undefined
来源
Archives of Virology | 2013年 / 158卷
关键词
Field Strain; Porcine Epidemic Diarrhea Virus; Infectious Bronchitis Virus; Porcine Epidemic Diarrhea Virus Strain; Predict Phosphorylation Site;
D O I
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中图分类号
学科分类号
摘要
Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006–2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.
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页码:1397 / 1401
页数:4
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