Classification and fatty acid composition analysis of Cronobacter spp. isolated from powdered infant formula in China

被引：0
作者：
Xiaojuan Yang
Qingping Wu
Jumei Zhang
Weipeng Guo
Shuping Mo
Shengrong Liu
机构：
[1] Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application
来源：
Food Science and Biotechnology | 2016年 / 25卷
关键词：
powdered infant formula; classification; cellular fatty acids;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study aimed to classify a collection of Enterobacter sakazakii (E. sakazakii) strains previously identified from powdered infant formula (PIF) to species level by recN gene sequencing and biochemical testing to determine the distribution of Cronobacter species in China and investigate the strain diversity by cellular fatty acid (CFA) analysis. Of 24 E. sakazakii isolates, 23 were identified as C. sakazakii and one was C. malonaticus. The 23 C. sakazakii isolates showed the same CFA profiles. The C. malonaticus isolate was discriminated from the C. sakazakii isolates by the significant difference in the amounts of C12:0, C14:0, and C17:0 cyclo acids. These results showed that C. sakazakii and C. malonaticus were the common Cronobacter species distributed in PIF in China and that the isolates of the two species exhibited different CFA profiles. These findings are of value for epidemiological investigations and provide an alternative method for confirming various Cronobacter spp.
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页码：1109 / 1113
页数：4
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