Binding Interaction of Xanthoxylin with Bovine Serum Albumin

被引：0
作者：
Mao-Gui Wen
Xin-Bo Zhang
Jian-Niao Tian
Shou-Hai Ni
He-Dong Bian
Yong-Lin Huang
Hong Liang
机构：
[1] Ministry of Education of China,Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University)
[2] Guangxi Normal University,Chemistry and Chemical Engineering
[3] Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences,Guangxi Institute of Botany
来源：
Journal of Solution Chemistry | 2009年 / 38卷
关键词：
Bovine serum albumin; Xanthoxylin; Conformational change; Energy transfer;
D O I：
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学科分类号：
摘要：
Three independent techniques have been used to investigate the interaction between bovine serum albumin (BSA) and xanthoxylin (XT). UV-Vis absorption spectroscopy measurements showed that there is a XT-BSA complex formed with an overall binding constant of K=1.01×105 L⋅mol−1. Spectroscopic techniques including synchronous fluorescence and Fourier transform infrared (FT-IR) were used to assess the structural effects of XT binding on BSA. The FT-IR experiments showed that there is a decrease of the amount of α-helix from 50.2 to 48.1% and an increase of the β-sheet from 32.9 to 36.9% in the XT-BSA complex. In addition, XT binds to site I of the protein with a distance of 2.07 nm between tryptophan residues and XT.
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[1]  
Roesler R.(2007)Antioxidant activity of Annona crassiflora: characterization of major components by electrospray ionization mass spectrometry Food Chem. 104 1048-1054
[2]  
Catharino R.R.(1986)Synthesis and antimicrobial activity of a series of caespitin derivaties Antimicrob. Agents Chemother. 30 375-381
[3]  
Malta L.G.(1993)Effects of extracts of Phytother. Res. 7 41-48
[4]  
Eberlin M.N.(1990) fruit and their constituents on spontaneous beating rate of myocardial cell sheets in culture Planta Med. 56 31-35
[5]  
Pastore G.(1994)Action of 2-hydroxy-4, 6-dimethoxy aceopheone isolated from Sebastiania schottiana Adv. Protein Chem. 45 153-203
[6]  
Van der Schye C.J.(2002)Structure of serum albumin J. Mol. Struct. 614 227-232
[7]  
Dekker T.G.(2005)Interaction of drugs with bovine and human serum albumin Bioorg. Med. Chem. 13 6609-6614
[8]  
Snyckers F.O.(2009)Interaction of cromolyn sodium with human serum albumin: A fluorescence quenching study Chin. J. Chem. 27 227-234
[9]  
Huang X.-L.(2007)Interaction between xanthoxylin and bovine serum albumin Guihaia 28 139-141
[10]  
Kakiuchi N.(1993)Study on the chemical constituents of blumea (I) Anal. Biochem. 213 407-413
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