Comparison of cytotoxicity effects induced by four different types of nanoparticles in human corneal and conjunctival epithelial cells

被引:0
作者
Xiangzhe Li
Boram Kang
Youngsub Eom
Jingxiang Zhong
Hyung Keun Lee
Hyo Myung Kim
Jong Suk Song
机构
[1] Korea University College of Medicine,Department of Ophthalmology, Guro Hospital
[2] First Affiliated Hospital of Jinan University,Department of Ophthalmology
[3] Sixth Affiliated Hospital of Jinan University,Department of Ophthalmology
[4] Yonsei University College of Medicine,Institute of Vision Research, Department of Ophthalmology
来源
Scientific Reports | / 12卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
The impact of particulate matter (PM) on ocular surface health has attracted increased attention in recent years. Previous studies have reported that differences in the chemical composition of PM can affect the toxicological response. However, available information on the toxic effects of chemical components of PM on the ocular surface is insufficient. In this paper, we aimed to investigate the toxicity effects of chemical components of PM on the ocular surface, focusing on the effects of four different types of nanoparticles (NPs) in human corneal epithelial cells (HCECs) and human conjunctival epithelial cells (HCjECs), which include titanium dioxide (TiO2), carbon black (CB), zinc dioxide (ZnO), and silicon dioxide (SiO2). We found that the in vitro cytotoxic effects of CB, ZnO, and SiO2 NPs are dependent on particle properties and cell type as well as the exposure concentration and time. Here, the order of increasing toxicity was SiO2 → CB → ZnO, while TiO2 demonstrated no toxicity. Moreover, toxic effects appearing more severe in HCECs than HCjECs. Reactive oxygen species (ROS)-mediated oxidative stress plays a key role in the toxicity of these three NPs in HCECs and HCjECs, leading to apoptosis and mitochondrial damage, which are also important contributors to aging. Sirtuin1 (SIRT1) as an NAD+-dependent protein deacetylase that seems to play a potential protective role in this process. These findings implied that ROS and/or SIRT1 may become a potential target of clinical treatment of PM- or NP-related ocular surface diseases.
引用
收藏
相关论文
共 121 条
  • [51] Yang J(2015)NiO nanoparticles induce apoptosis through repressing SIRT1 in human bronchial epithelial cells Toxicol. Appl. Pharmacol. 286 undefined-undefined
  • [52] Gao ZX(undefined)undefined undefined undefined undefined-undefined
  • [53] Lopez-Otin C(undefined)undefined undefined undefined undefined-undefined
  • [54] Blasco MA(undefined)undefined undefined undefined undefined-undefined
  • [55] Partridge L(undefined)undefined undefined undefined undefined-undefined
  • [56] Serrano M(undefined)undefined undefined undefined undefined-undefined
  • [57] Kroemer G(undefined)undefined undefined undefined undefined-undefined
  • [58] Tien CP(undefined)undefined undefined undefined undefined-undefined
  • [59] Zhao X(undefined)undefined undefined undefined undefined-undefined
  • [60] Chen C(undefined)undefined undefined undefined undefined-undefined