Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease

被引：0
作者：
Alejandra Larsen
Ester Teresa Gonzalez
María Soledad Serena
María Gabriela Echeverría
Eduardo Mortola
机构：
[1] Faculty of Veterinary Sciences,Veterinary Immunology
[2] University of La Plata,Virology
[3] UNLP,undefined
[4] Faculty of Veterinary Sciences,undefined
[5] University of La Plata,undefined
[6] Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET),undefined
来源：
Molecular Biotechnology | 2013年 / 54卷
关键词：
Bovine leukemia virus; Immunodetection; Baculovirus system; Recombinant p24 protein; Immunoblotting assay;
D O I：
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中图分类号：
学科分类号：
摘要：
Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
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页码：475 / 483
页数：8
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