Design of molecular markers for identification of species of the genus Chironomus (Diptera, Chironomidae)

被引：0

作者：

Gunderina L.I. ^{[1
]}

机构：

[1] Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk

来源：

Entomological Review | 2014年 / 94卷 / 1期

基金：

俄罗斯基础研究基金会;

关键词：

Polymerase Chain Reaction; Internal Transcribe Space; Entomological Review; Target Species; Sibling Species;

D O I：

10.1134/S0013873814010151

中图分类号：

学科分类号：

摘要：

Accurate identification and differentiation of species of the genus Chironomus based on their morphological features is a difficult problem. Unambiguous species identification by means of molecular markers is possible at any stage of the life cycle. Polymerase chain reaction (PCR) with species-specific primers was used to develop molecular markers (amplicons) for identification of Chironomus piger, Ch. dorsalis, and Ch. pseudothummi. Nucleotide sequences of the internal transcribed spacer region (ITS) of the locus coding for ribosomal RNA were used to design species-specific primers for these target species. Each of the species-specific primer pairs yielded species-specific amplicons (molecular markers) only with the DNA of target species: Ch. piger, Ch. dorsalis, and Ch. pseudothummi. Test PCRs with the DNA of eighteen Chironomus species confirmed the specificity of the primers obtained. The molecular markers produced in PCR with the designed species-specific primers permit reliable identification of Ch. piger, Ch. dorsalis, and Ch. pseudothummi and their differentiation from other species of the genus Chironomus. © 2014 Pleiades Publishing, Inc.

引用

页码：140 / 148

页数：8

共 30 条

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