Fluorescence Enhancement Effect for the Determination of Polychlorinated Biphenyls with Bovine Serum Albumin

被引：0
作者：
Fengju Zhang
Xia Wu
Jinhua Zhan
机构：
[1] Shandong University,Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering
来源：
Journal of Fluorescence | 2011年 / 21卷
关键词：
Fluorescence; Polychlorinated biphenyls; Bovine serum albumin; Determination;
D O I：
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中图分类号：
学科分类号：
摘要：
A sensitive and selective method for the trace determination of 3, 3’, 4, 4’-tetrachlorobiphenyl (PCB77) by using bovine serum albumin (BSA) as a fluorescence probe was introduced. Under optimum conditions, the enhanced fluorescence intensity was proportional to the concentration of polychlorinated biphenyls in the range of 8.9 × 10−8–5.0 × 10−6 mol L−1 for PCB77, and 5.0 × 10−7–5.0 × 10−6 mol L−1 for 2, 2’, 5, 5’-tetrachlorbiphenyl (PCB52). The detection limits (S/N = 3) of PCB77 and PCB52 were 2.6 × 10−8 mol L−1 and 2.9 × 10−7 mol L−1, respectively. Furthermore, the fluorescence enhancement mechanism was discussed in detail. Results indicated that fluorescence enhancement of the system originated from the formation of BSA-PCBs complexes. In addition, PCBs were mainly bound to the tyrosine residues in BSA molecules.
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