In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration

被引：81
作者：
Kasaj A. [1 ]
Reichert C. [1 ]
Götz H. [2 ]
Röhrig B. [3 ]
Smeets R. [4 ]
Willershausen B. [1 ]
机构：
[1] Department of Operative Dentistry and Periodontology, Johannes Gutenberg University, Mainz
[2] Institute of Applied Structure and Microanalysis, Medical Faculty, Johannes Gutenberg University, Mainz
[3] Institute for Medical Biostatistics, Epidemiology and Informatics, Johannes Gutenberg University, Mainz
[4] Department of Oral and Maxillofacial Surgery, Aachen University
来源：
Head & Face Medicine | / 4卷 / 1期
关键词：
Collagen Membrane; Gingival Fibroblast; Human Gingival Fibroblast; Guide Tissue Regeneration; Alamar Blue Assay;
D O I：
10.1186/1746-160X-4-22
中图分类号：
学科分类号：
摘要：
Background. Different types of bioabsorbable and nonresorbable membranes have been widely used for guided tissue regeneration (GTR) with its ultimate goal of regenerating lost periodontal structures. The purpose of the present study was to evaluate the biological effects of various bioabsorbable and nonresorbable membranes in cultures of primary human gingival fibroblasts (HGF), periodontal ligament fibroblasts (PDLF) and human osteoblast-like (HOB) cells in vitro. Methods. Three commercially available collagen membranes [TutoDent® (TD), Resodont® (RD) and BioGide® (BG)] as well as three nonresorbable polytetrafluoroethylene (PTFE) membranes [ACE (AC), Cytoplast® (CT) and TefGen-FD® (TG)] were tested. Cells plated on culture dishes (CD) served as positive controls. The effect of the barrier membranes on HGF, PDLF as well as HOB cells was assessed by the Alamar Blue fluorometric proliferation assay after 1, 2.5, 4, 24 and 48 h time periods. The structural and morphological properties of the membranes were evaluated by scanning electron microscopy (SEM). Results. The results showed that of the six barriers tested, TD and RD demonstrated the highest rate of HGF proliferation at both earlier (1 h) and later (48 h) time periods (P < 0.001) compared to all other tested barriers and CD. Similarly, TD, RD and BG had significantly higher numbers of cells at all time periods when compared with the positive control in PDLF culture (P ≤; 0.001). In HOB cell culture, the highest rate of cell proliferation was also calculated for TD at all time periods (P < 0.001). SEM observations demonstrated a microporous structure of all collagen membranes, with a compact top surface and a porous bottom surface, whereas the nonresorbable PTFE membranes demonstrated a homogenous structure with a symmetric dense skin layer. Conclusion. Results from the present study suggested that GTR membrane materials, per se, may influence cell proliferation in the process of periodontal tissue/bone regeneration. Among the six membranes examined, the bioabsorbable membranes demonstrated to be more suitable to stimulate cellular proliferation compared to nonresorbable PTFE membranes. © 2008 Kasaj et al; licensee BioMed Central Ltd.
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共 26 条
[1]  
Gottlow J., Guided tissue regeneration using bioresorbable and non-resorbable devices: Initial healing and long-term results, J Periodontol, 64, 11 SUPPL., pp. 1157-1165, (1993)
[2]  
Tatakis D.N., Promsudthi A., Wikesjo U.M., Devices for periodontal regeneration, Periodontol 2000, 19, pp. 59-73, (1999)
[3]  
Teparat T., Solt C.W., Claman L.J., Beck F.M., Clinical comparison of bioabsorbable barriers with non-resorbable barriers in guided tissue regeneration in the treatment of human intrabony defects, J Periodontol, 69, pp. 632-641, (1998)
[4]  
Giardino R., Fini M., Nicoli Aldini N., Giavaresi G., Rocca M., Polylactide bioabsorbable polymers for guided tissue regeneration, J Trauma-Injury Infection Crit Care, 47, pp. 303-308, (1999)
[5]  
Cortellini P., Prato G.P., Tonetti M.S., Periodontal regeneration of human intrabony defects with bioresorbable membranes. a controlled clinical trial, J Periodontol, 67, pp. 217-223, (1996)
[6]  
Weigel C., Bragger U., Hammerle C.H., Mombelli A., Lang N.P., Maintenance of new attachment 1 and 4 years following guided tissue regeneration (GTR), J Clin Periodontol, 22, pp. 661-669, (1995)
[7]  
MacHtei E.E., Grossi S.G., Dunford R., Zambon J.J., Genco R.J., Long-term stability of Class II furcation defects treated with barrier membranes, J Periodontol, 67, pp. 523-527, (1996)
[8]  
Selvig K.A., Kersten B.G., Chamberlain A.D., Wikesjo U.M., Nilveus R.E., Regenerative surgery of intrabony periodontal defects using ePTFE barrier membranes: Scanning electron microscopic evaluation of retrieved membranes versus clinical healing, J Periodontol, 63, pp. 974-978, (1992)
[9]  
Lorenzoni M., Pertl C., Keil C., Wegscheider W.A., Treatment of peri-implant defects with guided bone regeneration: A comparative clinical study with various membranes and bone grafts, Int J Oral Maxillofac Implants, 13, pp. 639-646, (1998)
[10]  
Minabe M., Kodama T., Kogou T., Tamura T., Hori T., Watanabe Y., Miyata T., Different cross-linked types of collagen implanted in rat palatal gingiva, J Periodontol, 60, pp. 35-43, (1989)
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