Influence of gold nanoparticles on collagen fibril morphology quantified using transmission electron microscopy and image analysis

被引：8
作者：
Haidekker M.A. [1 ]
Boettcher L.W. [1 ]
Suter J.D. [1 ]
Rone R. [1 ]
Grant S.A. [1 ]
机构：
[1] University of Missouri-Columbia, Deparment of Biological Engineering, Columbia
来源：
BMC Medical Imaging | / 6卷 / 1期
关键词：
Gold Nanoparticles; Collagen Fibril; Medial Axis; Control Image; Functionalized Gold Nanoparticles;
D O I：
10.1186/1471-2342-6-4
中图分类号：
学科分类号：
摘要：
Background: Development of implantable biosensors for disease detection is challenging because of poor biocompatibility of synthetic materials. A possible solution involves engineering interface materials that promote selfassembly and adhesion of autologous cells on sensor surfaces. Crosslinked type-1 collagen is an acceptable material for developing engineered basement membranes. In this study, we used functionalized gold nanoparticles as the crosslinking agent. Functionalized nanoparticles provide sites for crosslinking collagen as well as sites to deliver signaling compounds that direct selfassembly and reduce inflammation. The goal of this study was to obtain a quantitative parameter to objectively determine the presence of crosslinks. Methods: We analyzed TEM images of collagen fibrils by two methods: Run length analysis and topology analysis after medial axis transform. Results: Run length analysis showed a significant reduction of the interfibril spaces in the presence of nanoparticles (change of 40%, P < 0.05), whereas the fibril thickness remained unchanged. In the topological network, the number of elements, number of branches and number of sides increased significantly in the presence of nanoparticles (P < 0.05). Other parameters, especially the number of loops showed only a minimal and nonsignificant change. We chose a ratiometric parameter of the number of branches normalized by the number of loops to achieve independence from gross fibril density. This parameter is lower by a factor of 2.8 in the presence of nanoparticles (P < 0.05). Conclusion: The numerical parameters presented herein allow not only to quantify fibril mesh complexity and crosslinking, but also to help quantitatively compare cell growth and adhesion on collagen matrices of different degree of crosslinking in further studies. © 2006 Haidekker et al; licensee BioMed Central Ltd.
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  • [1] Sigler M., Handt S., Seghaye M.C., von Bernuth G., Grabitz R.G., Evaluation of in vivo biocompatibility of different devices for interventional closure of the patent ductus arteriosus in an animal model, Heart, 83, pp. 570-573, (2000)
  • [2] Pesakova V., Smetana Jr. K., Balik K., Hruska J., Petrtyl M., Hulejova H., Adam M., Biological and biochemical properties of the carbon composite and polyethylene implant materials, J Mater Sci Mater Med, 14, pp. 531-537, (2003)
  • [3] Feugier P., Black R.A., Hunt J.A., How T.V., Attachment, morphology and adherence of human endothelial cells to vascular prosthesis materials under the action of shear stress, Biomaterials, 26, pp. 1457-1466, (2005)
  • [4] Duan X., Sheardown H., Crosslinking of collagen with dendrimers, J Biomed Mater Res A, 75, pp. 510-518, (2005)
  • [5] Wissink M.J., van Luyn M.J., Beernink R., Dijk F., Poot A.A., Engbers G.H., Beugeling T., van Aken W.G., Feijen J., Endothelial cell seeding on crosslinked collagen: Effects of crosslinking on endothelial cell proliferation and functional parameters, Thromb Haemost, 84, pp. 325-331, (2000)
  • [6] Teixeira A.I., Abrams G.A., Bertics P.J., Murphy C.J., Nealey P.F., Epithelial contact guidance on well-defined micro- and nanostructured substrates, J Cell Sci, 116, pp. 1881-1892, (2003)
  • [7] Wen X., Shi D., Zhang N., Applications of nanotechnology in tissue engineering, Handbook of Nanostructural Biomaterials and Their Applications, 1, pp. 1-23, (2005)
  • [8] Schneider A., Francius G., Obeid R., Schwinte P., Hemmerle J., Frisch B., Schaaf P., Voegel J.C., Senger B., Picart C., Polyelectrolyte multilayers with a tunable Young's modulus: Influence of film stiffness on cell adhesion, Langmuir, 22, pp. 1193-1200, (2006)
  • [9] Bischofs I.B., Schwarz U.S., Cell organization in soft media due to active mechanosensing, Proc Natl Acad Sci USA, 100, pp. 9274-9279, (2003)
  • [10] Nishi K.K., Jayakrishnan A., Preparation and In Vitro Evaluation of Primaquine-Conjugated Gum Arabic Microspheres, Trends Biomater Artif Organs, 18, pp. 191-197, (2005)
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