Enhanced antibacterial effect against Enterococcus faecalis by silver ions plus Triton X-100 with low concentrations and cytotoxicity

被引:0
作者
Mengting Duan
Qing Sun
Wei Fan
Bing Fan
机构
[1] Wuhan University,The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei
来源
Brazilian Journal of Microbiology | 2022年 / 53卷
关键词
Triton X-100; Silver; Antimicrobial drug resistance; Antibacterial agent;
D O I
暂无
中图分类号
学科分类号
摘要
Enterococcus faecalis (E. faecalis) is commonly considered to be one of chief culprits of secondary and persistent root canal infections. As antibiotic resistance has become a global issue, in order to reduce the use of antibiotics, metal ions have recently been widely used as an alternative. Silver ions (Ag+) have been proved to be a strong bactericide but with high cytotoxicity and discoloration property. Triton X-100 (TX-100) and Ag+ were co-used for the first time as a clinical intracanal medication to obtain both enhanced antibacterial effect and low cytotoxicity. The synergistic antibacterial effect of TX-100 + Ag+ was tested on both planktonic and biofilm-resident E. faecalis on dentine. And the cytotoxicity was tested on MC3T3-E1 cells. Results confirmed the antibacterial activity against both planktonic and biofilm-resident E. faecalis was dramatically improved after TX-100 incorporation. TX-100 and Ag+ mixture demonstrated a similar inhibitory effect as the 2% chlorhexidine (CHX), while the cytotoxicity was much lower than 2% CHX (p < 0.05). In conclusion, TX-100 + Ag+ mixture might be developed into a new effective intracanal medication as the 2% CHX.
引用
收藏
页码:161 / 169
页数:8
相关论文
共 161 条
  • [1] Stuart CH(2006)Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment J Endod 32 93-98
  • [2] Schwartz SA(1997)Alkaline stress response in Enterococcus faecalis: adaptation, cross-protection, and changes in protein synthesis Appl Environ Microbiol 63 812-814
  • [3] Beeson TJ(1998)The emergence of enterococci as a cause of nosocomial infection Br J Biomed Sci 55 149-156
  • [4] Owatz CB(2004)Virulence factors of Enterococcus faecalis: relationship to endodontic disease Crit Rev Oral Biol Med 15 308-320
  • [5] Flahaut S(2003)Pathogenic enterococci: new developments in the 21st century Cell Mol Life Sci 60 2622-2636
  • [6] Hartke A(2013)Chlorhexidine in endodontics Braz Dent J 24 89-102
  • [7] Giard JC(2011)Observing the solubilization of lipid bilayers by detergents with optical microscopy of GUVs J Phys Chem B 115 269-277
  • [8] Auffray Y(2016)The effect of sodium hypochlorite and chlorhexidine as irrigant solutions for root canal disinfection: a systematic review of clinical trials J Endod 42 527-532
  • [9] Hunt CP(1974)Chlorhexidine in dental practice J Clin Periodontol 1 143-152
  • [10] Kayaoglu G(2019)Resistance toward chlorhexidine in oral bacteria—is there cause for concern? Front Microbiol 10 587-58