Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm

[1] Jinek M(2012)A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity Science 337 816-821

[2] Chylinski K(2013)RNA-guided human genome engineering via Cas9 Science 339 823-826

[3] Fonfara I(2014)Development and applications of CRISPR-Cas9 for genome engineering Cell 157 1262-1278

[4] Hauer M(2016)Biology and applications of CRISPR systems: harnessing nature’s toolbox for genome engineering Cell 164 29-44

[5] Doudna JA(2015)Making the cut CRISPR genome-editing technology shows its power Science 350 1456-1457

[6] Charpentier E(2014)Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation Nat Biotechnol 32 1262-1267

[7] Mali P(2016)Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 Nat Biotechnol 34 184-191

[8] Yang L(2013)CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity Nucleic Acids Res 41 9584-9592

[9] Esvelt KM(2013)High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells Nat Biotechnol 31 822-826

[10] Aach J(2013)DNA targeting specificity of RNA-guided Cas9 nucleases Nat Biotechnol 31 827-832

[1] Jinek M(2012)A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity Science 337 816-821

[2] Chylinski K(2013)RNA-guided human genome engineering via Cas9 Science 339 823-826

[3] Fonfara I(2014)Development and applications of CRISPR-Cas9 for genome engineering Cell 157 1262-1278

[4] Hauer M(2016)Biology and applications of CRISPR systems: harnessing nature’s toolbox for genome engineering Cell 164 29-44

[5] Doudna JA(2015)Making the cut CRISPR genome-editing technology shows its power Science 350 1456-1457

[6] Charpentier E(2014)Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation Nat Biotechnol 32 1262-1267

[7] Mali P(2016)Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 Nat Biotechnol 34 184-191

[8] Yang L(2013)CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity Nucleic Acids Res 41 9584-9592

[9] Esvelt KM(2013)High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells Nat Biotechnol 31 822-826

[10] Aach J(2013)DNA targeting specificity of RNA-guided Cas9 nucleases Nat Biotechnol 31 827-832