Rapid and stable in vitro regeneration of plants through callus morphogenesis in two varieties of Mucuna pruriens L. - An anti Parkinson's drug yielding plant

被引：7

作者：

Kotisree Lahiri

Madhumita J. Mukhopadhyay

Yves Desjardins

Sandip Mukhopadhyay

机构：

[1] Centre of Advanced Study, Department of Botany, University of Calcutta, Kolkata 700019, 35, Ballygunge Circular Road

[2] Department of Biotechnology, Institute of Genetic Engineering, BADU, Kolkata 700128, 30, Thakurhat Road

[3] Centre de recherche en horticulture, Pavillon de l'Envirotron, Université Laval, Québec

来源：

The Nucleus | 2012年 / 55卷 / 1期

关键词：

Callus morphogenesis; Chromosome; Genetic fidelity; ISSR; Mucuna pruriens; Plant regeneration; RAPD;

D O I：

10.1007/s13237-012-0051-7

中图分类号：

学科分类号：

摘要：

Mucuna pruriens L. is an important medicinal plant reported to contain L-3,4-dihydroxy phenylalanine (L-DOPA). The present report describes a simple protocol for plant regeneration through callus morphogenesis in four strains belonging to two different varieties of Mucuna pruriens. Friable, soft and nodular callus was induced from nodal and internodal segments of in vitro-grown seedlings on modified Murashige and Skoog's (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). For regeneration of shoots, the callus tissues were transferred to medium supplemented with various levels of cytokinin. Highest shoot regeneration from the callus tissue was achieved in modified MS medium fortified with BAP at 1.33 μM level. The regenerated shoots were rooted in vitro in half-strength of liquid MS medium supplemented with various levels of NAA. The regenerates were acclimatized for 2-3 weeks and showed about 80 % survival rate after transferring to the field. Cytological analysis revealed chromosome number stability of all the regenerates (2n022) with complete absence of aneuploidy. Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) analyses of total genomic DNA supported stability at the molecular level among all the clones of these strains when compared with their mother plants. © Archana Sharma Foundation of Calcutta 2012.

引用

页码：37 / 43

页数：6

共 32 条

[11]

Lahiri K., Mukhopadhyay M.J., Mukhopadhyay S., Karyotype analysis and in situ 4 C nuclear DNA quantification in two varieties of Mucuna pruriens L, J Trop Med Plants, 11, pp. 219-225, (2010)

[12]

Lahiri K., Mukhopadhyay M.J., Mukhopadhyay S., Enhancement of L-DOPA production in micropropagated plants of two different varieties of Mucuna pruriens L., available in India, Plant Tiss Cult Biotech, 21, pp. 115-125, (2011)

[13]

Lahiri K., Mukhopadhyay M.J., Mukhopadhyay S., Simple and efficient plant regeneration following somatic embryogenesis in Mucuna pruriens var. pruriens, a natural source of L-DOPA, J Trop Med Plants, (2012)

[14]

Lahiri K., Mukhopadhyay S., Mukhopadhyay M.J., A new report on induction of embryogenic callus culture in Mucuna pruriens L., an important medicinal plant, J Bot Soc Beng, 60, pp. 1-4, (2006)

[15]

Lee S.Y., Kim Y.K., Uddin M.R., Park N.I., Park S.U., An efficient protocol for shoot organogenesis and plant regeneration of buckwheat (Fagopyrum esculentum Moench.), Rom Biotech Let, 14, pp. 4524-4529, (2009)

[16]

May R.A., Sink K.C., Genotype and auxin influence direct somatic embryogenesis from protoplast derived from embryogenic cell suspensions of Asparagus officinalis L, Plant Sci, 108, pp. 71-84, (1995)

[17]

Mukhopadhyay M.J., Lahiri K., Mukhopadhyay S., In vitro microtuberization and enhanced colchicine accumulation in two species of Gloriosa, Cytologia, 73, pp. 357-363, (2008)

[18]

Mukhopadhyay M.J., Mukhopadhyay S., Sen S., In vitro propagation of Iphigenia indica, an alternative source of colchicine, Plant Cell Tiss Org Cult, 69, pp. 101-104, (2002)

[19]

Mukhopadhyay S., Banerjee N., Chromosome study and karyotype analysis in Solanum sarrachoides Sendt. A new report, Cytologia, 54, pp. 179-182, (1989)

[20]

Mukhopadhyay S., Desjardins Y., A comparative study on plant regeneration from protoplasts of two genotypes of Asparagus officinalis L, Plant Sci, 100, pp. 97-104, (1994)

← 1 2 3 4 →