Uptake of the dopaminergic neurotoxin, norsalsolinol, into PC12 cells via dopamine transporter

被引：0
作者：
Maruyama Y. [1 ]
Suzuki Y. [1 ]
Kazusaka A. [1 ]
Fujita S. [1 ]
机构：
[1] Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
来源：
Archives of Toxicology | 2001年 / 75卷 / 4期
关键词：
Dopamine transporter; Norsalsolinol; Parkinsonism; PC12; cells;
D O I：
10.1007/s002040000202
中图分类号：
学科分类号：
摘要：
The uptake of norsalsolinol, a neurotoxin candidate causing parkinsonism-like symptoms, was studied in PC12 cells. The compound was actively taken up by the PC12 cells, with a Km value of 176.2±9.1 μM and a maximum velocity of 55.6±7.0 pmol/min per mg protein; norsalsolinol uptake was dependent on the presence of extracellular Na+. The uptake of norsalsolinol was sensitive to two dopamine transporter inhibitors, GBR-12909 and reserpine, but was less sensitive to desipramine, a noradrenaline transporter inhibitor. Dopamine competitively inhibited norsalsolinol uptake into PC12 cells with a Ki value of 271.2±61.6 μM. These results suggest that norsalsolinol is taken up into PC12 cells mainly by the dopamine transporter.
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页码：209 / 213
页数：4
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