Thermophilic esterase from the archaeon Archaeoglobus fulgidus physically immobilized on hydrophobic macroporous resin: A novel biocatalyst for polyester synthesis

被引：0
作者：
Guangquan Li
Quanshun Li
机构：
[1] Jilin University,Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science
[2] Chinese Academy of Sciences,Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry
来源：
Biotechnology and Bioprocess Engineering | 2011年 / 16卷
关键词：
thermophilic esterase; immobilization; physical adsorption; ring-opening polymerization; ɛ-caprolactone;
D O I：
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学科分类号：
摘要：
This paper reviews the immobilization of a thermophilic esterase, AFEST from the archaeon Archaeoglobus fulgidus, on a hydrophobic macroporous resin and its application in polyester synthesis using the ring-opening polymerization of ɛ-caprolactone as a model. Using the physical adsorption technique, the AFEST loading concentration after 24 h was 152 mg AFEST per g of support. Particle size and surface morphology of the immobilized enzyme were investigated using laser scattering analysis and scanning electron microscopy. The effects of enzyme concentration, temperature, reaction time and reaction medium on monomer conversion and product molecular weight were systematically investigated. Through the optimization of reaction parameters, poly(ɛ-caprolactone) was obtained at an almost 100% monomer conversion rate and with a low average molecular weight (< 1,100 g/mol). Finally, the immobilized enzyme exhibited good operational stability, with a monomer conversion value of more than 55% after four batch reactions.
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页码：1201 / 1207
页数：6
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