Molecular cloning and characterization of a cyclin B gene on the ovarian maturation stage of black tiger shrimp (Penaeus monodon)

被引:0
作者
Lihua Qiu
Shigui Jiang
Falin Zhou
Jianhua Huang
Yihui Guo
机构
[1] Chinese Academy of Fishery Sciences,Biotechnology and aquiculture Laboratory, The South China Sea Fisheries Research Institute
来源
Molecular Biology Reports | 2023年 / 50卷
关键词
Cloning; Cyclin B; RT expression; Black tiger shrimp (; );
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中图分类号
学科分类号
摘要
The techniques of homology cloning and anchored PCR were used to clone the cyclin B gene from black tiger shrimp. The full length cDNA of black tiger shrimp cyclin B (btscyclin B) contained a 5′ untranslated region (UTR) of 102 bp, an ORF of 1,206 bp encoding a polypeptide of 401 amino acids with an estimated molecular mass of 45 kDa and a 3′ UTR of 396 bp. The searches for protein sequence similarities with BLAST analysis indicated that the deduced amino acid sequence of btscyclin B was homological to the cyclin B of other species and even the mammalians. Two conserved signature sequences of cyclin B gene family were found in the btscyclin B deduced amino acid sequence. The temporal expressions of cyclin B gene in the different tissues, including liver, ovary, muscle, brain stomach, heart and intestine, were measured by RT-PCR. mRNA expression of cyclin B could be detected in liver, ovary, muscle, brain, stomach, heart and strongest in the ovary, but almost not be detected in the intestine. In ovarian maturation stages, the expression of btscyclin B was different. The result indicated that btscyclin B was constitutive expressed and played an important role in the cell division stage.
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页码:S1 / S8
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