Induced Stress on Red Blood Cell Promotes Red Blood Cell-Endothelial Adhesion

被引：0

作者：

Vijayaraghavan M. ^{[1
,2
]}

Sengupta P. ^{[1
,2
]}

Sumantran V.N. ^{[3
]}

Suganya N. ^{[2
]}

Chatterjee S. ^{[1
,2
]}

机构：

[1] Department of Biotechnology, Anna University, Chennai

[2] AU-KBC Research Centre, Anna University, MIT Campus, Chennai

[3] APJ Kalam Center for Excellence, Dr. MGR Educational and Research Institute, Maduravoyal, Chennai

来源：

Cell and Tissue Biology | 2020年 / 14卷 / 6期

关键词：

Adhesion; cholesterol depletion; hypertonic shock; N-omega-nitro-L-arginine methyl ester (L‑NAME); nitric oxide;

D O I：

10.1134/S1990519X20060085

中图分类号：

学科分类号：

摘要：

Abstract: Besides disease condition, very few stress stimulants were determined to provoke red blood cell (RBC) adhesion to endothelial cells (EC). However, the possible role of other stress factors which disrupt RBCs anti-adhesive property is still unknown. To resolve this, we studied in vitro static adherence of RBC-EC after RBC exposure to physical (osmotic and shear stress) and chemical stress stimulants (cholesterol depletion and nitric oxide modulation). In support with earlier studies, RBC under hypertonic shock demonstrated a significant increase in RBC-EC adherence as a result of prominent structural modification. Besides, our study shows that shear stress, cholesterol depletion and nitric oxide inhibition in RBC increases RBC-EC adhesiveness which elucidates cholesterol, nitric oxide and shear stress importance in preventing RBC-EC adhesion. Thus, present study shows that assessment of RBC-EC interaction after exerting to external stress is critical in understanding pathophysiological conditions. © 2020, Pleiades Publishing, Ltd.

引用

页码：448 / 457

页数：9

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