Colorimetric determination of cysteine by exploiting its inhibitory action on the peroxidase-like activity of Au@Pt core-shell nanohybrids

被引：0
|
作者：
Na Pan
Wang Li-Ying
Liang-Liang Wu
Chi-Fang Peng
Zheng-Jun Xie
机构：
[1] Jiangnan University,State Key Lab of Food Science and Technology, School of Food Science and Technology
来源：
Microchimica Acta | 2017年 / 184卷
关键词：
Metal nanoparticles; Enzyme mimic; Electron paramagnetic resonance; Lineweaver-Burk diagram; Michaelies-Menten kinetics;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The authors report that the peroxidase-like activity of Au@Pt core-shell nanohybrids (Au@PtNHs) is selectively inhibited by cysteine. This finding has led to  a highly sensitive colorimetric assay for cysteine that is based on the nanohybrid-catalyzed oxidation of TMB by H2O2 to form a blue product. The method has a detection limit of 5.0 nM and a linear range from 10 nM to 20 μM. The assay is highly selective over other amino acids. It was successfully applied to the determination of cysteine in an injection containing a mixture of amino acids.
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页码：65 / 72
页数：7
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