The application of solid-phase extraction and reversed phase high-performance liquid chromatography for simultaneous isolation and determination of plant flavonoids and phenolic acids

A new method combining solid-phase extraction and reversed phase high-performance liquid chromatography was developed for simultaneous isolation and determination of flavones (baicalin, baicalein, chrysin, scutellarein) and some phenolic acids in aerial and underground parts of a medicinal plant—Scutellaria baicalensis Georgi. The application of optimised enrichment conditions, elaborated on octadecyl and quaternary amine BakerBond microcolumns have led to the extraction of both groups of analytes with recoveries >95% and variation coefficients <5%.