Micropropagation of Dianthus caryophyllus L. — Control of Vitrification

被引:0
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作者
Amita Jain
S. Husain
S. L. Kothari
机构
[1] University of Rajasthan,Department of Botany
关键词
Normal Shoot; Adventitious Shoot Regeneration; Organogenic Callus; Shoot Morphogenesis; Dianthus Caryophyllus;
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学科分类号
摘要
Vitrification is a morphological and physiological disorder affecting in vitro regenerated plants. Vitrified shoots of carnation (Dianthus caryophyllus L.) regenerated from cultured cotyledons were abnormally glassy, thick and bushy with wider translucent leaves. Such vitrified shoots were recovered by culturing them on a medium supplemented with GA3. Differentiation of shoot buds from the cultured cotyledons of D. caryophyllus occurred on MS medium supplemented with BAP (2.0 mg/l). Shoot buds subcultured on the same medium resulted in further prolific development of shoot buds and bushy shoot growths. Key words: carnation, shoot morphogenesis, micropropagation, cotyledons vitrification.
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页码:35 / 37
页数:2
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